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Purification, characterization and specificity of a new GH family 35 galactosidase from Aspergillus awamori.

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Relation http://ir.cftri.com/14662/
https://doi.org/10.1016/j.ijbiomac.2020.04.013 0141-8130/
 
Title Purification, characterization and specificity of a new GH family 35
galactosidase from Aspergillus awamori.
 
Creator Vidya, C. H.
Gnanesh Kumar, B. S.
Chinmayee, Cirium V.
Sridevi Annapurna, Singh
 
Subject 04 Fermentation Technology
05 Enzymes
 
Description Galactosidases, ubiquitous in nature, are complex carbohydrate-active enzymes and find extensive applications in food, pharma, and biotechnology industries. The present study deals with the production of galactosidases from fungi by solid-state fermentation. Fifteen fungi were screened and Aspergillus awamori
(MTCC 548), exhibited the highest α and β-galactosidase activities of 75.11±0.29 U/g and 155.34±
1.26 U/g, respectively. 30 g of wheat bran substituted with 6% defatted soy flour, at 28°C, pH 5.0 for
120 h, was established as the optimum production conditions by one-factor approach. The enzyme was purified to homogeneity with an apparent mass of 118 ± 2 kDa by ammonium sulfate precipitation (50–80%),
ion exchange and hydrophobic interaction chromatography. Specific activities for α and β-galactosidase
were 22 and 74 U/mg, respectively. Optimum temperature and pH ranges for enzyme activities were
55–60 °C, 5.0–5.5, respectively. The thermal inactivation mid-point was 65 °C. The purified enzyme not
only exhibited α and β-galactosidase activities, but also exhibited β-xylosidase and β-glucosidase activities,
indicating the enzyme has broad substrate specificity. Sequence analysis by in-gel digestion and tandem
mass spectrometry (MS/MS) revealed that the enzyme was a probable β-galactosidase A, belonging to glycoside hydrolase 35 family, and is being reported for the first time.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14662/1/International%20Journal%20of%20Biological%20Macromolecules%20156%20%282020%29%20885%E2%80%93895.pdf
Vidya, C. H. and Gnanesh Kumar, B. S. and Chinmayee, Cirium V. and Sridevi Annapurna, Singh (2020) Purification, characterization and specificity of a new GH family 35 galactosidase from Aspergillus awamori. International Journal of Biological Macromolecules, 156. pp. 885-895. ISSN 0141-8130