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Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice.

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Relation http://ir.cftri.com/14668/
https://doi.org/10.1016/j.jaim.2017.10.006 0975-9476/
 
Title Humoral immune and adjuvant responses of mucosally-administered
Tinospora cordifolia immunomodulatory protein in BALB/c mice.
 
Creator Aranha, Ivan
Venkatesh, Y. P.
 
Subject 10 Plants
03 Proteins
 
Description Background: In traditional medicine, guduchi (Tinospora cordifolia) is considered as an adaptogen with
immunomodulatory prowess. A 25 kDa protein from guduchi stem has been characterized as an
immunomodulatory protein (ImP).
Objectives: The aim of this study was to evaluate the intrinsic immunogenicity of guduchi ImP and
adjuvant activity using ovalbumin (OVA) as antigen in BALB/c mice.
Materials and Methods: Mice were given guduchi ImP (30 and 60 mg) by intranasal administration to
respective groups (n ΒΌ 6) on days 1, 14 and thereafter weekly till day 42. Immunogenic response was
monitored by serum IgG/IgA levels (days 14, 35 and 50). The adjuvant activity was measured by serum
anti-OVA IgG/IgA responses to administration of 30 mg OVA with guduchi ImP. The effect of guduchi ImP
on the spleen status was examined by splenic weight (day 50).
Results: Guduchi ImP administration displayed a significant increase in anti-guduchi ImP IgG (5e7 fold)
and anti-guduchi ImP IgA (3e4 fold) on day 50 vs. control. Guduchi ImP showed a significant increase in
anti-OVA IgG (6e7 fold) and anti-OVA IgA (4e5 fold) on day 50 vs. control. The splenic index of guduchi
ImP group increased significantly in both the immune and adjuvant response groups; however, the
splenic index in the adjuvant response group was markedly higher.
Conclusion: The results indicate that guduchi ImP is a strong immunogen by itself and enhances the
immunogenicity of mucosally-administered antigen in BALB/c mice. Based on the results of this animal
study, it appears that guduchi ImP shows a potential for future studies in humans.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14668/1/Journal%20of%20Ayurveda%20and%20Integrative%20Medicine%2011%20%282020%29%20140e146.pdf
Aranha, Ivan and Venkatesh, Y. P. (2020) Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice. Journal of Ayurveda and Integrative Medicine, 11. pp. 140-146.