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Application of Optimized and Validated Agar Overlay TLC–Bioautography Assay for Detecting the Antimicrobial Metabolites of Pharmaceutical Interest.

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Relation http://ir.cftri.com/14670/
https://doi.org/10.1093/chromsci/bmaa045
 
Title Application of Optimized and Validated Agar
Overlay TLC–Bioautography Assay for Detecting
the Antimicrobial Metabolites of Pharmaceutical
Interest.
 
Creator Bettadapura Rameshgowda, Nuthan
Devaraju, Rakshith
Kuppuru Mallikarjunaiah, Marulasiddaswamy
Yashavantha Rao, H.C.
Ramesha, Kolathur Puttamadaiah
Chandra Mohana, Nagabhushana
Siddappa, Shiva
Darshan, Dorairaj
 
Subject 10 Plants
06 Chromatography
 
Description The agar overlay TLC–bioautography is one of the crucial methods for simultaneous in situ
detection and separation of antimicrobial metabolites of pharmaceutical interest. The main focus
of this research relies on the dereplication of an antimicrobial metabolite coriloxin derived from
mycoendophytic Xylaria sp. NBRTSB-20 with a validation of agar overlay TLC–bioautography technique. This polyketide metabolite coriloxin was purified by column chromatography, and its purity
was assessed by HPLC, UPLC-ESI-QTOF-MS, FT-IR and NMR spectral analysis. The antimicrobial
capability of ethyl acetate extract and the purified compound coriloxin was determined by disc
diffusion, minimal inhibitory concentration and agar overlay TLC–bioautography assay. The visible
LOD of coriloxin antimicrobial activity was found at 10 µg for Escherichia coli and 20 µg for both
Staphylococcus aureus and Fusarium oxysporum. Inter- and intra-day precision was determined as
the relative standard deviation is less than 6.56%, which proved that this method was precise. The
accuracy was expressed as recovery, and the values were found ranging from 91.18 to 108.73% with
RSD values 0.94–2.30%, respectively. The overall findings of this investigation suggest that agar
overlay TLC–bioautography assay is a suitable and acceptable method for the in situ determination
of antimicrobial pharmaceuticals
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14670/1/Journal%20of%20Chromatographic%20Science%2C%202020%2C%201%E2%80%9310.pdf
Bettadapura Rameshgowda, Nuthan and Devaraju, Rakshith and Kuppuru Mallikarjunaiah, Marulasiddaswamy and Yashavantha Rao, H.C. and Ramesha, Kolathur Puttamadaiah and Chandra Mohana, Nagabhushana and Siddappa, Shiva and Darshan, Dorairaj (2020) Application of Optimized and Validated Agar Overlay TLC–Bioautography Assay for Detecting the Antimicrobial Metabolites of Pharmaceutical Interest. Journal of Chromatographic Science. pp. 1-10.