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Hyperglycemia impairs osteoblast cell migration and chemotaxis due to a decrease in mitochondrial biogenesis.

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Relation http://ir.cftri.com/14677/
https://doi.org/10.1007/s11010-020-03732-8
 
Title Hyperglycemia impairs osteoblast cell migration and chemotaxis due
to a decrease in mitochondrial biogenesis.
 
Creator Heena, Pahwa
Touseef Khan, Md.
Kunal, Sharan
 
Subject 13 Molecular Biochemistry
04 Diabetes Mellitus
 
Description Diabetes is associated with an increase in skeletal fragility and risk of fracture. However, the underlying mechanism for the
same is not well understood. Specifcally, the results from osteoblast cell culture studies are ambiguous due to contradicting
reports. The use of supraphysiological concentrations in these studies, unachievable in vivo, might be the reason for the
same. Therefore, here, we studied the efect of physiologically relevant levels of high glucose during diabetes (11.1 mM)
on MC3T3-E1 osteoblast cell functions. The results showed that high glucose exposure to osteoblast cells increases their
diferentiation and mineralization without any efect on the proliferation. However, high glucose decreases their migratory
potential and chemotaxis with a decrease in the associated cell signaling. Notably, this decrease in cell migration in high
glucose conditions was accompanied by aberrant localization of Dynamin 2 in osteoblast cells. Besides, high glucose also
caused a shift in mitochondrial dynamics towards the appearance of more fused and lesser fragmented mitochondria, with a
concomitant decrease in the expression of DRP1, suggesting decreased mitochondrial biogenesis. In conclusion, here we are
reporting for the frst time that hyperglycemia causes a reduction in osteoblast cell migration and chemotaxis. This decrease
might lead to an inefcient movement of osteoblasts to the erosion site resulting in uneven mineralization and skeletal fragility found in type 2 diabetes patients, in spite of having normal bone mineral density (BMD).
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14677/1/Molecular%20and%20Cellular%20Biochemistry%20%282020%29%20469109%E2%80%93118.pdf
Heena, Pahwa and Touseef Khan, Md. and Kunal, Sharan (2020) Hyperglycemia impairs osteoblast cell migration and chemotaxis due to a decrease in mitochondrial biogenesis. Molecular and Cellular Biochemistry, 469. pp. 109-118. ISSN 0300-8177