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Colorimetric and chemiluminescence based enzyme linked apta-sorbent assay (ELASA) for ochratoxin A detection.

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Relation http://ir.cftri.com/14682/
https://doi.org/10.1016/j.saa.2020.118875 1386-1425/
 
Title Colorimetric and chemiluminescence based enzyme linked apta-sorbent
assay (ELASA) for ochratoxin A detection.
 
Creator Monali, Mukherjee
Nandhini, C.
Praveena, Bhatt
 
Subject 01 Analysis
10 Food Microorganisms
 
Description Ochratoxin A (OTA) is one of the most widespread mycotoxin found to contaminate various food products such
as cereals, spices, groundnuts, coffee, wine, beer etc. It is also carried over from contaminated feed and fodder to
milk, blood, meat, kidney and liver of animals consuming it. Enzyme-linked to biorecognition molecules like antibodies or aptamers are very popular due to their ability to be used as labels or tags in biosensing formats. In this
work, OTA aptamer based colorimetric and chemiluminescence biosensing formats were evaluated for the detection of OTA. The colorimetric enzyme linked apta-sorbent assay (Co-ELASA) and chemiluminescence enzyme
linked apta-sorbent assay (Cl-ELASA) showed a linear detection range from 1 pg/mL to 1 μg/mL with a limit of
detection (LOD) of 0.84 pg/mL for Co-ELASA (limit of quantification (LOQ) = 2.54 pg/mL) and 1.29 pg/mL for
Cl-ELASA (LOQ = 3.94 pg/mL) under optimized buffer conditions. Comparison of ELASA methods with sandwich
ELISA indicated that the developed techniques had sensitivity similar to the conventional technique which indicated a LOD of 1.13 pg/mL and LOQ of 3.41 pg/mL. Studies in simulated contaminated food samples by spiking
OTA in groundnut and coffee bean at concentrations of 0.1, 1 and 10 ppb, indicated recoveries in the range of
50.21 to 113.27% for Co-ELASA, 90.47 to 107.72% for Cl-ELASA and 76.23 to 141.49% for ELISA. Results of the
study indicate that Co-ELASA and Cl-ELASA assays could be an alternate approach for ultrasensitive detection
of OTA in food samples, which can also be adapted for biosensor development.
 
Date 2021
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14682/1/Spectrochimica%20Acta%20Part%20A%20Molecular%20and%20Biomolecular%20Spectroscopy%20244%20%282021%29%20118875.pdf
Monali, Mukherjee and Nandhini, C. and Praveena, Bhatt (2021) Colorimetric and chemiluminescence based enzyme linked apta-sorbent assay (ELASA) for ochratoxin A detection. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 244. p. 118875.