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Efect of biotic and abiotic elicitors on isofavone biosynthesis during seed development and in suspension cultures of soybean (Glycine max L.).

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Relation http://ir.cftri.com/14684/
https://doi.org/10.1007/s13205-020-2065-1
 
Title Efect of biotic and abiotic elicitors on isofavone biosynthesis
during seed development and in suspension cultures of soybean
(Glycine max L.).
 
Creator Akitha Devi, M. K.
Gyanendra, Kumar
Giridhar, P.
 
Subject 04 Biosynthesis
05 Soya bean
 
Description The present investigation aimed to look at the efects of biotic and abiotic elicitors during Soybean seed development and
cell suspension culture in isofavones accumulation. The expression levels of four major genes viz., CHS7, CHS8, IFS2,
and IFS1 involved on isofavones biosynthesis during seed developmental stages from R5L–R7 was seen in both MAUS-2
and JS-335 Soybean varieties. The R7 stage showed 1.24-fold upregulation of IFS1transcript level and considered as the
control for Soybean seed development. Both varieties during R6−R8 stages responded diferently to the foliar application of
10 µM SA, 10 µM MJ and 0.1% Aspergillus niger. The IFS2 transcripts were upregulated by SA at the R7 stage with 5.21-
and 4.68-fold in JS-335 and MAUS-2, respectively. IFS1 expression was signifcantly increased by A. niger treatment at R7
stage with 3.98- and 3.21-fold in MAUS-2 and JS-335, respectively. The expression of CHS7 and CHS8 by 10 μM SA at R7
level revealed maximum up-regulation of 0.51- and 1.01-fold in MAUS-2; 0.37- and 0.82-fold in JS-335, respectively. In the
soybean callus suspension culture, biosynthetic genes were used to validate the efects of elicitor on isofavones. Both biotic
and abiotic treatments contribute to the upregulation of IFS1 and IFS2 expression, that in turn, leads to the accumulation of
isofavone in seed development as well as in suspension cultures. These data further suggested that the IFS2 is the key gene
responsible for the isofavone accumulation during elicitor treatment.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14684/1/3%20Biotech%20%282020%29%201098.pdf
Akitha Devi, M. K. and Gyanendra, Kumar and Giridhar, P. (2020) Efect of biotic and abiotic elicitors on isofavone biosynthesis during seed development and in suspension cultures of soybean (Glycine max L.). 3 Biotech, 10. p. 98.