Record Details

Metal Ion–Enhanced Quantification of Chloramphenicol in Milk Using Imipramine Hydrochloride as Diazo-Coupling Agent.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/14688/
https://doi.org/10.1007/s12161-020-01837-w
 
Title Metal Ion–Enhanced Quantification of Chloramphenicol in Milk Using
Imipramine Hydrochloride as Diazo-Coupling Agent.
 
Creator Richa, Sharma
Honnur, Krishna
Raghavarao, K. S. M. S.
 
Subject 02 Safety
27 Dairy products
 
Description Chloramphenicol is a broad-spectrum antibiotic that is administered to cattle, fish, poultry, and honeybees among other animals.
Consumption of food from such sources leads to non-regenerative anaemia, aplastic anaemia, and bone marrow suppression in
humans. It is therefore a pressing necessity to detect chloramphenicol in food items through a convenient, accurate, and rapid
method. The proposed method is the first attempt of a diazo-coupling reaction between diazotized chloramphenicol and imipramine hydrochloride yielding an intense purple-coloured product at room temperature, with λmax = 575 nm. The concentration
of reagents, temperature, and acid medium were optimised. Enhancement of molar absorptivity coefficient of the dye by a range
of different salts and nanoparticles, and their effect on reaction kinetics were also studied in details for the first time. Iron (III) was
seen to show highest catalytic efficiency. The increase in molar absorptivity coefficient for the dye product was calculated to be
from 0.14 to 0.2 μM−1 cm−1
. The catalysed reaction was employed for determination of chloramphenicol. The linear range was
from 0.2135 to 20 ppb and detection limit for CAP was 2.321 ppb. This method is simple, sensitive, and economical without the
need of specific reaction conditions or dedicated sophisticated instrumentation.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14688/1/Food%20Analytical%20Methods%20%282020%29%20132321%E2%80%932329.pdf
Richa, Sharma and Honnur, Krishna and Raghavarao, K. S. M. S. (2020) Metal Ion–Enhanced Quantification of Chloramphenicol in Milk Using Imipramine Hydrochloride as Diazo-Coupling Agent. Food Analytical Methods, 13. pp. 2321-2329.