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Wheat bran oil concentrate induced AMPK activation, insulin and lipid homeostasis alleviates adipokines and cytokine in high fat fed C57BL6 mice.

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Relation http://ir.cftri.com/14699/
https://doi.org/10.1016/j.jafr.2020.100080
 
Title Wheat bran oil concentrate induced AMPK activation, insulin and lipid
homeostasis alleviates adipokines and cytokine in high fat fed C57BL6 mice.
 
Creator Sharanappa, T. T.
Mohan Kumar, A. S.
Bhaskaragoud, G.
Mohan Kumar, B. V.
Suresh Kumar, G.
 
Subject 04 Diabetes Mellitus
19 Lipids-oils/fats
04 Wheat
 
Description The mechanism of fat soluble bioactives on activation of AMPK, normalization of insulin and serum proteome on
obesity is not yet clear. This study investigated the effect of wheat bran oil concentrate on adipokines, proinflammatory markers and transcription factors in relation to differentially expressed serum proteins using
two-dimensional electrophoresis (2DE) approach-in high fat diet (HFC) fed C57BL6 mice.
The HFC group exhibited a significant increase in body weight, insulin level and lipid profile. The ELISA and
Western blot analysis of serum proteins of HFC group also exhibited increased level of inflammatory markers
(TNF-α, TGF-β and interleukin-1β), tissue transcription factors (SREBP-2 & PPARγ) and were validated by gene
expression and immunohistochemistry. However, the treatment with WBA 1 and 2% significantly lessened lipid
droplets in 3T3-L1 cells. Reduced insulin level, down-regulated transcription factors and associated inflammatory
cytokines by activating AMPK in HFC-fed mice. Furthermore, WBA treatment directly reversed differentially
expressed recombinant proteins of KCTD family and Zinc finger protein 236 isoform.
Bioactive fraction of WBA had beneficial effect on ameliorating obesity related complications mainly through
phosphorylation of AMPK and inflammatory cytokines which was authenticated by proteomic analysis
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14699/1/Journal%20of%20Agriculture%20and%20Food%20Research%202%20%282020%29%20100080.pdf
Sharanappa, T. T. and Mohan Kumar, A. S. and Bhaskaragoud, G. and Mohan Kumar, B. V. and Suresh Kumar, G. (2020) Wheat bran oil concentrate induced AMPK activation, insulin and lipid homeostasis alleviates adipokines and cytokine in high fat fed C57BL6 mice. Journal of Agriculture and Food Research, 2. p. 100080.