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Alkali-stable GH11 endo-b-1,4 xylanase (XynB) from Bacillus subtilis strain CAM 21: application in hydrolysis of agro-industrial wastes, fruit/vegetable peels and weeds.

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Relation http://ir.cftri.com/14706/
https://doi.org/10.1080/10826068.2020.1830416
 
Title Alkali-stable GH11 endo-b-1,4 xylanase (XynB) from Bacillus subtilis strain CAM
21: application in hydrolysis of agro-industrial wastes, fruit/vegetable peels
and weeds.
 
Creator Monica, P.
Mukesh, Kapoor
 
Subject 05 Enzymes
07 Waste utilization
 
Description GH11 endo-xylanases, due to their inherent structural and biochemical properties, are the key to
efficient bioconversion of lignocellulosic biomass into value-added products. A GH11 endo-xylanase (XynB) from Bacillus subtilis strain CAM 21 was cloned, over-expressed and purified
(Mw�24 kDa) using Ni-NTA affinity chromatography. XynB showed optimum activity at pH 7.0 and
50C and was stable (>88%) in a broad range of pH (4–11). The apparent Km, Kcat and Kcat/Km of
XynB were 2.9 mg/ml, 1961.2/sec, and 675.62 ml/mg/sec, respectively using birchwood xylan as
substrate. XynB was a classical endo-xylanase as it hydrolyzed birchwood xylan to xylo-oligosaccharides and not xylose. Kinetic stability of XynB at 45–53C was between 43-182 min. Secondary
structure analysis of XynB using far-UV CD spectroscopy revealed presence of 51.85% b strands
and 2.64% a helix and was consistent with the homology modeling studies. XynB hydrolyzed the
xylan extracted from agro-industrial wastes and fruit/vegetable peels by releasing up to 670 mg/g
of reducing sugars. The xylan extracted from weeds (Ageratum conyzoides, Achyranthes aspera and
Tridax procumbens) had characteristic signatures of hemicelluloses and after XynB hydrolysis
showed cracks, peeling and release of up to 135.2 mg/g reducing sugars.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14706/1/Preparative%20Biochemistry%20%26%20Biotechnology%2C%202020.pdf
Monica, P. and Mukesh, Kapoor (2020) Alkali-stable GH11 endo-b-1,4 xylanase (XynB) from Bacillus subtilis strain CAM 21: application in hydrolysis of agro-industrial wastes, fruit/vegetable peels and weeds. Preparative Biochemistry & Biotechnology.