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An efficient, high-throughput method for the simultaneous exposure of drought stress and bacterial infection in plants

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Title An efficient, high-throughput method for the simultaneous exposure of drought stress and bacterial infection in plants
 
Creator Choudhary, Aanchal
Senthil-Kumar, Muthappa
 
Subject Arabidopsis thaliana
bacteria
combined stress
drought
Pseudomonas syringae
 
Description Accepted date: 11 September 2020
PREMISE: We developed a systematic protocol for the easy, high-throughput, qualitative, and
quantitative assessment of the patho-morphological, physiological, and molecular responses
of Arabidopsis thaliana plants simultaneously subjected to drought and bacterial infection.
This approach will assist studies elucidating plant adaptation strategies to combat combined
stresses.
METHODS AND RESULTS: Plants were grown in small screw-capped containers, individual
pots, or pot strips. Watering was withheld from 30-day-old plants, which were subsequently
infected with Pseudomonas syringae pv. tomato DC3000 using a dip inoculation. The natural
development of both drought and bacterial infection was successfully and rapidly replicated
in large numbers of plants, which is difficult to achieve with existing protocols.
CONCLUSIONS: Our protocol offers a simple, low-cost, high-throughput strategy for the rapid
and easy bacterial infection of large numbers of plants. It can be used in large-scale mutant
and ecotype screenings under combined stresses and for other foliar pathogens in different
plant species.
This work was supported by National Institute of Plant Genome
Research (New Delhi, India) core funding, a Department of
Biotechnology Ramalingaswami re-entry fellowship to M.S-K. (BT/RLF/reentry/23/2012), and a Senior Research Fellowship to A.C.
(DBT/2014/NIPGR/261). The authors thank Mr. Nilesh Sharma and
Mr. Rahim Tarafdar (National Institute of Plant Genome Research)
for their technical help with the experiments. We acknowledge the
DBT-eLibrary Consortium for providing access to e-resources, and
the NIPGR Plant Growth Facility for providing growth space and
maintenance. We thank Dr. Mahesh Patil, Mr. Vadivel Murugan
Irulappan, and Ms. Urooj Fatima (National Institute of Plant
Genome Research) for internally reviewing the manuscript.
 
Date 2020-12-07T09:27:30Z
2020-12-07T09:27:30Z
2020
 
Type Article
 
Identifier Applications in Plant Sciences, 8(11): e11399
2168-0450
https://doi.org/10.1002/aps3.11399
https://bsapubs.onlinelibrary.wiley.com/doi/full/10.1002/aps3.11399
http://223.31.159.10:8080/jspui/handle/123456789/1130
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons