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COP1 mediates light-dependent regulation of flavonol biosynthesis through HY5 in Arabidopsis
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View Archive Info| Field | Value | |
| Title |
COP1 mediates light-dependent regulation of flavonol biosynthesis through HY5 in Arabidopsis
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| Creator |
Bhatia, Chitra
Gaddam, Subhash Reddy Pandey, Ashutosh Trivedi, Prabodh Kumar |
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| Subject |
COP1
Flavonol HY5 Light-dependent regulation MYB transcription factors |
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| Description |
Accepted date: 13 November 2020
Flavonols, a class of flavonoids, accumulate as protective agents in response to various stresses. Among various environmental stimuli, light is one of the factors regulating flavonol production. MYB12/11/111, members of the R2R3 MYBs family, regulates spatio-temporal flavonol accumulation in Arabidopsis. Although various studies indicate at the involvement of an E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and ELONGATED HYPOCOTYL 5 (HY5) in flavonoid biosynthesis in response to UV-B, the regulatory roles of these components under visible light are yet to be investigated. Here, we demonstrate that flavonol accumulation in Arabidopsis is light-regulated. Furthermore, our analysis suggests that MYB12 is a HY5-dependent light-inducible gene and plays a key role in the activation of the flavonol biosynthesis in response to light. Our results indicate the involvement of COP1 in the dark-dependent repression of MYB12 expression and flavonol accumulation. In addition, results also suggest that the effect of COP1 on MYB12 is indirect and is mediated through HY5, a direct transcriptional activator of the MYB12. Together these findings indicate that COP1 acts as a master negative regulator of flavonol biosynthesis in the dark. We thank Prof. Ute Hoecker for providing hy5, cop1-4 and hy5cop1-4 mutant seeds. Also COP1-BD and COP1-AD vectors were provided by her. We also acknowledge Dr.Gabriela Toledo-Ortiz for providing pifQ seeds. Research was supported by Council of Scientific and Industrial Research, New Delhi in the form of Network Project (GAP-3400) and CSIR-BMBF collaborative project. C.B. and S.R.G. acknowledge University Grant Commission, New Delhi for Senior and Junior Research Fellowship respectively. |
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| Date |
2020-12-07T09:39:27Z
2020-12-07T09:39:27Z 2021 |
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| Type |
Article
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| Identifier |
Plant Science, 303: 110760
0168-9452 https://doi.org/10.1016/j.plantsci.2020.110760 https://www.sciencedirect.com/science/article/pii/S0168945220303666 http://223.31.159.10:8080/jspui/handle/123456789/1131 |
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| Language |
en_US
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| Format |
application/pdf
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| Publisher |
Elsevier B.V.
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