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Wheat 2-Cys peroxiredoxin plays a dual role in chlorophyll biosynthesis and adaptation to high temperature

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Title Wheat 2-Cys peroxiredoxin plays a dual role in chlorophyll biosynthesis and adaptation to high temperature
 
Creator Mishra, Divya
Shekhar, Shubhendu
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Antioxidant defense
Bread wheat
Cell death suppressors
Cytoplasmic proteome
High temperature stress
Stress-adaptive responses
Thermotolerance
 
Description Accepted date: 07 December 2020
The molecular mechanism of high temperature stress (HTS) response, in plants, has so far been investigated using transcriptomics, while the dynamics of HTS‐responsive proteome remain unexplored. We examined the adaptive responses of the resilient wheat cultivar ‘Unnat Halna’ and dissected the HTS‐responsive proteome landscape. This led to the identification of 55 HTS‐responsive proteins (HRPs), which are predominantly involved in metabolism and defense pathways. Interestingly, HRPs included a 2‐cysteine peroxiredoxin (2CP), designated Ta2CP, presumably involved in stress perception and adaptation. Complementation of Ta2CP in yeast and heterologous expression in Arabidopsis demonstrated its role in thermotolerance. Both Ta2CP silencing and overexpression inferred the involvement of Ta2CP in plant growth and chlorophyll biosynthesis. We demonstrated that Ta2CP interacts with protochlorophyllide reductase b, TaPORB. Reduced TaPORB expression was found in Ta2cp‐silenced plants, while upregulation was observed in Ta2CP‐overexpressed plants. Furthermore, the downregulation of Ta2CP in Taporb‐silenced plants and reduction of protochlorophyllide in Ta2cp‐silenced plants suggested the key role of Ta2CP in chlorophyll metabolism. Additionally, the transcript levels of AGPase1 and starch were increased in Ta2cp‐silenced plants. More significantly, HTS‐treated Ta2cp‐silenced plants showed adaptive responses despite increased reactive oxygen species and peroxide concentrations, which might help in rapid induction of high‐temperature acclimation.
This work was supported by Grants from the Council of Scientific and Industrial Research (CSIR)
(38(1487)/19/EMR-II), Govt. of India. The authors kindly acknowledge the University Grant
Commission (UGC), Govt. of India for providing predoctoral fellowship to D.M. and DST-SERB
(PDF/2016/001417) for providing postdoctoral fellowship to S.S. The authors thank Prof. Dong-Yan
Jin for the generous gift of the yeast mutants, and Prof. Martin J. Muller and Dr. Francisco Javier
Cejudo for the Arabidopsis double mutants, and Dr. Manoj Prasad for the tomato PDS-VIGS
construct. The authors are thankful to AIRF, Jawaharlal Nehru University, New Delhi, India, for GCMS and TEM analyses. The authors also acknowledge the assistance of DBT-eLibrary consortium
(DeLCON) for making literature available.
 
Date 2020-12-09T10:23:36Z
2020-12-09T10:23:36Z
2020
 
Type Article
 
Identifier Plant Journal, (In Press)
1365-313X
https://doi.org/10.1111/tpj.15119
https://onlinelibrary.wiley.com/doi/10.1111/tpj.15119
http://223.31.159.10:8080/jspui/handle/123456789/1133
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons