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Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum

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Relation http://oar.icrisat.org/11667/
https://doi.org/10.3390/plants9121727
doi:10.3390/plants9121727
 
Title Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum
 
Creator Gopalakrishnan, S
Sharma, R
Srinivas, V
Naresh, N
Mishra, S P
Ankati, S
Pratyusha, S
Govindaraj, M
Gonzalez, S V
Nervik, S
Simic, N
 
Subject Sorghum
Plant Disease
 
Description Streptomycesalbus strain CAI-21 has been previously reported to have plant growth-promotion
abilities in chickpea, pigeonpea, rice, and sorghum. The strain CAI-21 and its secondary metabolite
were evaluated for their biocontrol potential against charcoal rot disease in sorghum caused
by Macrophomina phaseolina. Results exhibited that CAI-21 significantly inhibited the growth of
the pathogen, M. phaseolina, in dual-culture (15 mm; zone of inhibition), metabolite production
(74% inhibition), and blotter paper (90% inhibition) assays. When CAI-21 was tested for its biocontrol
potential under greenhouse and field conditions following inoculation of M. phaseolina by toothpick
method, it significantly reduced the number of internodes infected (75% and 45% less, respectively)
and length of infection (75% and 51% less, respectively) over the positive control (only M. phaseolina
inoculated) plants. Under greenhouse conditions, scanning electron microscopic analysis showed
that the phloem and xylem tissues of the CAI-21-treated shoot samples were intact compared to
those of the diseased stem samples. The culture filtrate of the CAI-21 was purified by various
chromatographic techniques, and the active compound was identified as “organophosphate” by
NMR and MS. The e�cacy of organophosphate was found to inhibit the growth of M. phaseolina
in the poisoned food technique. This study indicates that S. albus CAI-21 and its active metabolite
organophosphate have the potential to control charcoal rot in sorghum.
 
Publisher MDPI
 
Date 2020-12
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11667/1/Identification%20and%20Characterization%20CAI-21%20metabolite-SGK%20et%20al.%202020.pdf
Gopalakrishnan, S and Sharma, R and Srinivas, V and Naresh, N and Mishra, S P and Ankati, S and Pratyusha, S and Govindaraj, M and Gonzalez, S V and Nervik, S and Simic, N (2020) Identification and Characterization of a Streptomyces albus Strain and Its Secondary Metabolite Organophosphate against Charcoal Rot of Sorghum. Plants (TSI), 9 (12). pp. 1-14. ISSN 2223-7747