Antioxidant, antimicrobial and cytotoxic activity of Curkolin® (Curcuma longa and Coleus forskohlii formulation)
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Title |
Antioxidant, antimicrobial and cytotoxic activity of Curkolin® (Curcuma longa and Coleus forskohlii formulation)
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Creator |
Sadashiva, C T
Hussain, H M Firoz Nanjundaiah, S Manjula, A C Kote, NV Patil, R Ranjith Makeswari, M Ravi, S |
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Subject |
Antioxidant
Antimicrobial Curkolin® HPLC MTT assay |
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Description |
751-756
The aim of this study was to screen the in vitro antimicrobial, antioxidant and cytotoxicity effect of Curkolin®, a combination of curcumin and C. forskolin in the ratio 4:1. Curcuma longa L. is the source of curcumin while C. forskohlii has been used in traditional ayurvedic medicine for curing various disorders and therefore the forskolin is the source of the diterpenoids. The medicinal properties of plants are investigated throughout the world for scientific advancement for their important pharmacological activities, user convenient, economically viable with low toxicity. The antioxidant property of Curkolin® was determined to be 55.42 µg/ml using (Diphenyl picryl hydrazine) DPPH assay and was then compared with standard Butylated hydroxytoluene (BHT). The antimicrobial activity was assessed by calculating the MIC and MBC using microplate serial dilution technique. It exhibited 250 µg/ml, the least MIC value of Bacillus cereus as compared to the rest Gram-positive (Streptococcus mutans and Staphylococcus aureus) and Gram-negative (Escherichia coli and Salmonella typhi) bacteria. Further, it was analyzed for cytotoxic screening on MCF-7 breast cancer cell line by MTT assay, which has shown a strong anti-proliferative activity. The IC50 values of Curkolin® were found 135.8 μg/ml. Together these results suggest the combination to be an effective antioxidant, antimicrobial and cytotoxic agent. |
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Date |
2020-12-23T10:05:35Z
2020-12-23T10:05:35Z 2020-10 |
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Type |
Article
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Identifier |
0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscair.res.in/handle/123456789/55829 |
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Language |
en_US
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Relation |
Int. Cl.20: A61P 17/18, C12Q 1/18, A61K 8/97, A61K 38/00, C12Q 1/6839
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Rights |
CC Attribution-Noncommercial-No Derivative Works 2.5 India
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Publisher |
NISCAIR-CSIR, India
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Source |
IJTK Vol.19(4) [October 2020]
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