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Genome-wide identification, structure analysis and expression profiling of phospholipases D under hormone and abiotic stress treatment in chickpea (Cicer arietinum)

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Title Genome-wide identification, structure analysis and expression profiling of phospholipases D under hormone and abiotic stress treatment in chickpea (Cicer arietinum)
 
Creator Sagar, Sushma
Deepika
Biswas, Dipul Kumar
Chandrasekar, Ramsankar
Singh, Amarjeet
 
Subject Phospholipase D
Abiotic stress
Hormone
Gene expression
Protein localization
Chickpea
 
Description Accepted date: 13 December 2020
Phospholipases D (PLDs) are phospholipid hydrolyzing enzymes and crucial components of lipid signaling in plants. PLDs are implicated in stress responses in different plants however, characterization of PLDs in chickpea is missing. Here, we identify 13 PLD genes in the chickpea genome. PLD family could be divided into α, β, δ, ε and ζ isoforms based on sequence and structure. Protein remodeling described that chickpea PLDs are composed of defined arrangements of α-helix, β-sheets and short loops. Phylogenetic analysis suggested evolutionary conservation of chickpea PLD family with dicots. In-planta subcellular localization showed the plasma membrane localization of chickpea PLDs. All PLD promoters had hormone and stress related cis-regulatory elements, which suggested overlapping function of PLDs in hormone and abiotic stress signaling. The qRT-PCR expression analysis revealed that most PLD genes are differentially expressed in multiple abiotic stresses (drought, salt and cold stress). Moreover, several PLD genes had overlapping expression in abiotic stress and ABA and JA treatment. These observations indicate the involvement of PLD gene family in cross-talk of phytohormone and abiotic stress signaling in chickpea. Thus, present study opens new avenues of utilizing PLD related information for understanding hormone-regulated abiotic stress signaling in legume crops.
DBT (Department of Biotechnology)- eLibrary Consortium
(DeLCON) is acknowledged for e-resources and National Institute of
Plant Genome Research (NIPGR) is acknowledged for financial support
from core research grant. SS and Deepika are thankful to council of
scientific and industrial research (CSIR), India for research fellowships. Research fund was provided by the National Institute of Plant
Genome Research (NIPGR), India core research grant.
 
Date 2020-12-22T09:21:40Z
2020-12-22T09:21:40Z
2021
 
Type Article
 
Identifier International Journal of Biological Macromolecules, 169: 264-273
0141-8130
https://doi.org/10.1016/j.ijbiomac.2020.12.102
https://www.sciencedirect.com/science/article/pii/S0141813020352703
http://223.31.159.10:8080/jspui/handle/123456789/1137
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.