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Phylogeography and Symbiotic Effectiveness of Rhizobia Nodulating Chickpea (Cicer arietinum L.) in Ethiopia

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Relation http://oar.icrisat.org/11680/
https://doi.org/10.1007/s00248-020-01620-8
doi:10.1007/s00248-020-01620-8
 
Title Phylogeography and Symbiotic Effectiveness of Rhizobia Nodulating Chickpea (Cicer arietinum L.) in Ethiopia
 
Creator Gunnabo, A H
van Heerwaarden, J
Geurts, R
Wolde-meskel, E
Degefu, T
Giller, K E
 
Subject Molecular Biology
Chickpea
Legume Crops
Ethiopia
 
Description Chickpea (Cicer arietinum L.) used to be considered a restrictive host that nodulated and fixed nitrogen only with Mesorhizobium
ciceri and M.mediterraneum. Recent analysis revealed that chickpea can also establish effective symbioseswith strains of several
other Mesorhizobium species such as M. loti, M. haukuii, M. amorphae, M. muleiense, etc. These strains vary in their nitrogen
fixation potential inviting further exploration. We characterized newly collected mesorhizobial strains isolated from various
locations in Ethiopia to evaluate genetic diversity, biogeographic structure and symbiotic effectiveness. Symbiotic effectiveness
was evaluated in Leonard Jars using a locally released chickpea cultivar “Nattoli”. Most of the new isolates belonged to a clade
related to M. plurifarium, with very few sequence differences, while the total collection of strains contained three additional
mesorhizobial genospecies associated with M. ciceri, M. abyssinicae and an unidentified Mesorhizobium species isolated from a
wild host in Eritrea. The four genospecies identified represented a subset of the eight major Mesorhizobium clades recently
reported for Ethiopia based on metagenomic data. All Ethiopian strains had nearly identical symbiotic genes that grouped them in
a single cluster with M. ciceri, M. mediterraneum and M. muleiense, but not with M. plurifarium. Some phylogeographic
structure was observed, with elevation and geography explaining some of the genetic differences among strains, but the relation
between genetic identity and symbiotic effectiveness was observed to be weak.
 
Publisher Springer
 
Date 2020-10
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11680/1/Gunnabo2020_Article_PhylogeographyAndSymbioticEffe.pdf
Gunnabo, A H and van Heerwaarden, J and Geurts, R and Wolde-meskel, E and Degefu, T and Giller, K E (2020) Phylogeography and Symbiotic Effectiveness of Rhizobia Nodulating Chickpea (Cicer arietinum L.) in Ethiopia. Microbial Ecology (TSI). ISSN 0095-3628