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<p><strong>Antibacterial and anti-HIV activity of extracellular pigment from <em>Streptomyces </em>sp. S45 isolated from Sabarimala forest soil, India</strong></p>

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Title Statement <p><strong>Antibacterial and anti-HIV activity of extracellular pigment from <em>Streptomyces </em>sp. S45 isolated from Sabarimala forest soil, India</strong></p>
 
Added Entry - Uncontrolled Name Vaishnavi, M
Manigundan, K
Smalia, Thania
Nandhini, S Usha
Gopikrishnan, V
Kumar, Ashok
Hanna, Luke Elizabeth
Radhakrishnan, M
Aruni, Wilson
 
Uncontrolled Index Term Actinobacteria; Antimicrobial; Forest ecosystem; Partial purification; Western Ghats
 
Summary, etc. Actinobacteria, particularly from under-studied habitats, are often endowed with diverse biological properties. In the present study, about 17 actinobacterial strains were isolated from Sabarimala forest an understudied ecosystem in Western Ghats, India and screened for their pigment producing potential. Pinkish brown soluble pigment producing <em>Streptomyces</em> sp. strain S45 was selected and screened for antibacterial and anti-HIV activity. The bioactivity of ethyl acetate extract of the strain S45 showed maximum zone of inhibition against <em>Staphylococcus</em><em> aureus</em> ATCC 29213 (17.3±0.4 mm) and <em>Bacillus cereus</em> (15.6±0.6 mm). Also, it showed anti-HIV activity with the IC50 value of 8.75 µg/mL. The bioactive pigment isolated from the strain S45 was partially purified and characterized using UV absorption. In bio-autography, an antibacterial compound found to be active against <em>S. aureus</em> ATCC 29213 and its MIC values ranged between 25-1.56 µg/mL. Variables such as glucose, rhamnose, soybean meal and CaCl<sub>2</sub>, pH 7 and temperature 30℃ were found to influence bioactive pigment production. Potential strain S45 was identified as <em>Streptomyces </em>species on the basis of microscopic, cultural, physiological and 16SrRNA analysis. Results suggest that the <em>Streptomyces</em> sp. S45 strain explored in this study could be a promising candidate for isolation of antibacterial and anti HIV pigment.
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-12-29 15:50:54
 
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http://op.niscair.res.in/index.php/IJEB/article/view/44575
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 12 (2020): IJEB [December 2020]
 
Language Note en