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Biological properties and structural characterization of a novel rhamnolipid like-biosurfactants produced by Lactobacillus casei subsp. casei TM1B.

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Relation http://ir.cftri.com/14709/
https://doi.org/10.1002/bab.1966
 
Title Biological properties and structural characterization of a novel rhamnolipid like-biosurfactants
produced by Lactobacillus casei subsp. casei TM1B.
 
Creator Hippolyte, T. Mouafo
Augustin, Mbawala
Somashekar, D.
Hervé, M. Tchougang
Nanishankar, V. Harohally
 
Subject 04 Fermentation Technology
 
Description Biosurfactants are microbial surface-active compounds with antimicrobial and antioxidant
activities that display a range of physiological functions. In this study, a strain isolated from a
Cameroonian fermented milk ―pendidam‖ and identified as Lactobacillus casei subsp. casei
TM1B was used for biosurfactants production. The biosurfactants produced by L. casei
TM1B with molasses as the substrate had a good surface (40.77 mN/m) and emulsifying
(84.50%) activities. The scavenging of the ABTS+• radical (IC50 value of 0.60 ± 0.03 mg/mL)
by the biosurfactants was found to be higher than that of DPPH• radical (IC50 value of 0.97 ±
0.13 mg/mL). The maximum chelating activity of biosurfactants (82.29%) was observed at
3.5 mg/mL. The biologically active compound of the biosurfactants produced by L. casei
TM1B was identified as 2,5-O-methylrhamnofuranosyl-palmitate, a novel rhamnolipid likebiosurfactant by using chemical, FTIR, gas chromatography-mass spectrometry, and NMR
analysis. The biosurfactants were bactericidal against several Gram-negative and Grampositive pathogens (MIC values ranged from 3.22 to 12.83 mg/mL), and SEM analysis
revealed bacterial cell walls and membranes as main targets.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14709/1/10.1002%40bab.1966.pdf
Hippolyte, T. Mouafo and Augustin, Mbawala and Somashekar, D. and Hervé, M. Tchougang and Nanishankar, V. Harohally (2020) Biological properties and structural characterization of a novel rhamnolipid like-biosurfactants produced by Lactobacillus casei subsp. casei TM1B. Botechnology and Applied Biochemistry.