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Simple click chemistry-based derivatization to quantify endogenous formaldehyde in milk using ultra-high-performance liquid chromatography tandem mass spectrometry in selected reaction monitoring mode.

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Relation http://ir.cftri.com/14710/
https:/doi.org/10.1002/rcm.8865
 
Title Simple click chemistry-based derivatization to quantify endogenous
formaldehyde in milk using ultra-high-performance liquid
chromatography tandem mass spectrometry in selected reaction
monitoring mode.
 
Creator Sweekruthi, A. Shetty
Rangiah, K.
 
Subject 06 Chromatography
27 Dairy products
 
Description RATIONALE Formaldehyde (FA) exposure via environmental pollution or through the food
chain poses serious threat to human health, especially in developing countries like India.
Although the addition of FA to food is proscribed, it is often illegally added to foods such as
milk to increase the shelf life. There are challenges in differentiating the endogenous FA
content in milk from externally added FA.
METHOD We have developed a simple UHPLC/MS/SRM method for the absolute
quantification of endogenous FA in milk. The steps include fat removal, protein precipitation
using acid, and spiking with labelled FA (FA*), followed by simple click chemistry-based
derivatization using Girard P (GP) reagent and final analysis.
RESULTS A standard curve with FA* was constructed and used for the calculation of
endogenous FA in milk. The optimal conditions for the derivatization reaction using 500 μL of
milk were: GP, 50 g; temperature, 37 C; time, 60 min; and 0.1% HCl. The validation
parameters such as accuracy (95.84 to 99.73%), precision (2.84 to 8.02%) and spiked recovery
(>95%) are within the FDA guidelines. This method is highly sensitive [limit of detection
(LOD) of 1 ng/mL] with a dynamic range of 3.12 to 200 ng/mL. The endogenous FA level in
pasteurized cow milk is 70 ng/mL (n=60). The FA content in raw milk samples from cow,
goat, buffalo (each n=10) varied from 134 to 255 ng/mL.
CONCLUSIONS This method is precise and sufficiently sensitive to quantify endogenous FA
in milk samples using a minimal sample volume. As it involves simple sample preparation
steps, it can be used routinely to quantify endogenous FA.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14710/1/10.1002%40rcm.8865.pdf
Sweekruthi, A. Shetty and Rangiah, K. (2020) Simple click chemistry-based derivatization to quantify endogenous formaldehyde in milk using ultra-high-performance liquid chromatography tandem mass spectrometry in selected reaction monitoring mode. Rapid Communications Mass Spectrometry, 34 (19). e8865.