Chitosan-sodium alginate-fatty acid nanocarrier system: Lutein bioavailability, absorption pharmacokinetics in diabetic rat and protection of retinal cells against H2O2 induced oxidative stress in vitro.
IR@CSIR-CFTRI
View Archive InfoField | Value | |
Relation |
http://ir.cftri.com/14717/
https://doi.org/10.1016/j.carbpol.2020.117409 |
|
Title |
Chitosan-sodium alginate-fatty acid nanocarrier system: Lutein bioavailability, absorption pharmacokinetics in diabetic rat and protection of retinal cells against H2O2 induced oxidative stress in vitro. |
|
Creator |
Veeresh, T.
Baskaran, V. |
|
Subject |
17 Fatty Acid Chemistry
28 Polysaccharide Chemistry |
|
Description |
Aiming to enhance therapeutic efficiency of lutein, lutein loaded chitosan-sodium alginate (CS-SA) based nanocarrier system (LNCs) were prepared and evaluated for lutein bioavailability and pharmacokinetics in diabetic rats in comparison to micellar lutein (control). Further, cytotoxicity, cellular uptake and protective activity against H2O2 induced oxidative stress in ARPE-19 cells were studied. Results revealed that LNCs displayed maximal lutein AUC in plasma, liver and eye respectively in normal (3.1, 2.7 and 5.2 folds) and diabetic (7.3, 3.4 and 2.8 folds) rats. Lutein from LNCs exhibited a higher half-life time, mean residence time and slow clearance from the plasma, indicating prolonged circulation compared to control. In ARPE-19 cells, pretreatment with LNCs (10 μM) have significantly attenuated H2O2 induced cell death, intracellular ROS and mitochondrial membrane potential compared to control. In conclusion, LNCs improve the lutein bioavailability in conditions like diabetes, diabetic retinopathy and cataract to curtail oxidative stress in retinal cells. |
|
Date |
2021
|
|
Type |
Article
PeerReviewed |
|
Format |
pdf
|
|
Language |
en
|
|
Identifier |
http://ir.cftri.com/14717/1/Carbohydrate%20Polymers%20254%20%282021%29%20117409.pdf
Veeresh, T. and Baskaran, V. (2021) Chitosan-sodium alginate-fatty acid nanocarrier system: Lutein bioavailability, absorption pharmacokinetics in diabetic rat and protection of retinal cells against H2O2 induced oxidative stress in vitro. Carbohydrate Polymers, 254. p. 117409. |
|