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<strong>Permethrin induced cytotoxicity of rat splenocytes: Protective effect of N-acetylcysteine</strong>

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Title Statement <strong>Permethrin induced cytotoxicity of rat splenocytes: Protective effect of N-acetylcysteine</strong>
 
Added Entry - Uncontrolled Name Ahmed, Tanzeel ; School of Biotechnology, IFTM University, Moradabad, Uttar Pradesh, India
Banerjee, Basu Dev
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Uncontrolled Index Term Permethrin, Splenocytes, Cytotoxicity, Apoptosis, N-acetylcysteine
 
Summary, etc. <p><strong>ABSTRACT</strong></p> <p>The present study was designed to determine the molecular mechanism associated with permethrin induce cytotoxicity. Rat splenocytes were incubated with increasing concentration of permethrin (0-39 ug/ml) for 6 to 24 h. Cytotoxic effect of permethrin was evaluated by MTT assay. To assess the underneath mechanism of cytotoxicity, different biochemical indices of cell death namely Annexin V binding assay, DNA fragmentation, and levels of caspase 3 were analyzed. To evaluate the oxidative stress, glutathione depletion and melondialdehyde levels were analyzed. MTT assay revealed that permethrin induces cytotoxicity in dose-dependent way. In Annexin-V binding assay, above 7.8 µg/ml concentration, significant necrosis of cells was noticed and consistent with DNA fragmentation assay. A significant dose and time dependent depletion of cellular glutathione (GSH) and increased MDA levels were observed and consistent with the percentage of cells undergoing apoptosis. Co administration of N-acetycysteine mitigates permethrin- induced apoptosis, showing the role of oxidative stress in apoptosis induction. The present study gives experimental evidence that emphasizing the role of oxidative stress in permethrin-induced cytotoxicity in rat splenocytes in vitro.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-12-30 15:56:05
 
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http://op.niscair.res.in/index.php/IJEB/article/view/33064
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 1 (2021): IJEB [January 2021]
 
Language Note en