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A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM
 
Creator Sharma, Sandeep
Muddassir, Mohd
Muthusamy, Saraladevi
Vaishnav, Pardeep Kumar
Singh, Manish
Sharma, Deepak
Kanagarajan, Selvaraju
Shanmugam, Vijayakumar
 
Subject QR Microbiology
 
Description Classical plant uptake is limited to hydrophilic or water-dispersible material. Therefore, in order to test the uptake behaviour of hydrophobic particles, here, we tested the fate of hydrophobic particles (oleylamine coated Cu2-xSe NPs (CS@OA)) in comparison to hydrophilic particles (chitosan-coated Cu2-xSe NPs (CS@CH)) by treatment on the plant roots. Surprisingly, hydrophobic CS@OA NPs have been found to be similar to 1.3 times more efficient than hydrophilic CS@CH NPs in tomato plant root penetration. An atomic force microscopy (AFM) adhesion force experiment confirms that hydrophobic NPs experience non-spontaneous yet energetically favorable root trapping and penetration. Further, a relative difference in the hydrophobic vs. hydrophilic NPs movement from roots to shoots has been observed and found related to the change in protein corona as identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) analysis. Finally, the toxicity assays at the give concentration showed that Cu2-xSe NPs lead to non-significant toxicity as compared to control. This technology may find an advantage in fertilizer application.
 
Publisher Nature publishing group
 
Date 2020-11-06
 
Type Article
PeerReviewed
 
Relation https://www.nature.com/articles/s41598-020-75685-3
http://crdd.osdd.net/open/2628/
 
Identifier Sharma, Sandeep and Muddassir, Mohd and Muthusamy, Saraladevi and Vaishnav, Pardeep Kumar and Singh, Manish and Sharma, Deepak and Kanagarajan, Selvaraju and Shanmugam, Vijayakumar (2020) A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM. Scientific reports, 10 (1). ISSN 2045-2322