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Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31
 
Creator Choukate, Komal
Gupta, Aanchal
Basu, Brohmomoy
Karman, Vrk
Ganguli, Munia
Chaudhuri, Barnali
 
Subject QR Microbiology
 
Description DivIVA or Wag31, which is an essential pole organizing protein in mycobacteria, can self-assemble at the negatively curved side of the membrane at the growing pole to form a higher order structural scaffold for maintaining cellular morphology and localizing various target proteins for cell-wall biogenesis. The structural organization of polar scaffold formed by polymerization of coiled-coil rich Wag31, which is implicated in the anti-tubercular activities of amino-pyrimidine sulfonamides, remains to be determined. A single-site phosphorylation in Wag31 regulates peptidoglycan biosynthesis in mycobacteria. We report biophysical characterizations of filaments formed by mycobacterial Wag31 using circular dichroism, atomic force microscopy and small angle solution X-ray scattering. Atomic force microscopic images of the wild-type, a phospho-mimetic (T73E) and a phospho-ablative (T73A) form of Wag31 show mostly linear filament formation with occasional curving, kinking and apparent branching. Solution X-ray scattering data indicates that the phospho-mimetic forms of the Wag31 polymers are on average more compact than their phospho-ablative counterparts, which is likely due to the extent of bending/branching. Observed structural features in this first view of Wag31 filaments suggest a basis for higher order Wag31 scaffold formation at the pole.
 
Publisher Elsevier Science
 
Date 2020-02
 
Type Article
PeerReviewed
 
Relation https://www.sciencedirect.com/science/article/pii/S1047847719302552#!
http://crdd.osdd.net/open/2630/
 
Identifier Choukate, Komal and Gupta, Aanchal and Basu, Brohmomoy and Karman, Vrk and Ganguli, Munia and Chaudhuri, Barnali (2020) Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. Journal of structural biology, 209 (2). ISSN 1095-8657