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Identification, isolation, and heterologous expression of Sunflower wax synthase for the synthesis of tailored wax esters.

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Relation http://ir.cftri.com/14739/
http://dx.doi.org/10.1111/jfbc.13433
 
Title Identification, isolation, and heterologous expression of
Sunflower wax synthase for the synthesis of tailored wax
esters.
 
Creator Shalini, Theresa
Asha, Martin
 
Subject 05 Enzymes
Sunflower Seed
 
Description Wax esters (WE) are neutral lipids formed by condensation of fatty alcohol with fatty
acyl-CoA by wax synthases. They serve as carbon and energy reserves and are potential substrates for various commercial applications. Sunflower (Helianthus annuus)
an edible oil seed is a source of WE, however, the gene responsible for WE formation has hitherto remained unidentified. Using an in silico approach we identified,
isolated putative Sunflower wax synthase (HaWS) gene and investigated it’s potential
for WE production in yeast. Heterologous expression of HaWS in Saccharomyces cerevisiae H1246 exhibited 57 kDa protein which was confirmed by immunoblotting.
Recombinant yeast expressing HaWS were fed with combinations of C16, C18 fatty
alcohols with 16:0, 18:0 fatty acyl CoA’s as potential substrates to validate WE formation in vivo. The yeast cells accumulated C-32 to C-36 WE. Our study reveals
identification, isolation, and heterologous functional expression of WS gene from
Sunflower for the first time.
Practical applications
Wax synthases (WSs) are critical enzymes for wax ester (WE) biosynthesis. WEs are
high value products having several industrial applications. WE serve as substrates
for lubricants, food coatings, cosmetics, and pharmaceuticals. There is a demand for
alternate renewable resource of WEs. In this study, we have successfully isolated
a putative wax synthase gene from Sunflower and submitted its sequence data to
the GenBank (Accession number MH460820). Conserved sequence search analysis
showed presence of condensation superfamily motif‒HHXXXDG, critical for WE biosynthesis. Heterologous expression of HaWS in yeast revealed synthesis of C-32 to
C-36 WE. Our study demonstrates the efficacy of HaWS to accumulate specific WE
of desired lengths in yeast, and thus represents an alternate source of WE for commercial applications and for biotechnological production of tailored WE in eukaryotic
expression systems.
 
Publisher Wiley
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14739/1/J%20Food%20Biochem.%202020%3B44e13433..pdf
Shalini, Theresa and Asha, Martin (2020) Identification, isolation, and heterologous expression of Sunflower wax synthase for the synthesis of tailored wax esters. Journal of Food Biochemistry. ISSN 1745-4514