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Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses

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Relation http://oar.icrisat.org/11688/
https://doi.org/10.1016/j.sajb.2020.06.011
doi:10.1016/j.sajb.2020.06.011
 
Title Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses
 
Creator Shinde, H
Dudhate, A
Anand, L
Tsugama, D
Gupta, S K
Liu, S
Takano, T
 
Subject Abiotic Stress
Plant Breeding
Pearl Millet
 
Description Pearl millet [Pennisetum glaucum (L.) R. Br.] is an important crop in arid and semi-arid areas of India and
Africa. It is well known for its tolerance to abiotic stresses, but it lags behind other cereals in terms of
research and development. MicroRNAs (miRNAs) are a versatile group of small regulatory RNAs of 20�22
bases that play important roles in plant growth, development, and stress responses. However, the regulatory
mechanisms underlying miRNA-mediated responses to salinity stress in pearl millet are still unclear. In this
study, we performed small RNA sequencing to identify conserved and novel miRNAs from the salinity tolerant
pearl millet genotype. In total, 130 million sequence reads were generated, and 81 conserved and 14
novel miRNAs were identified as salinity stress responsive microRNAs. We also performed target prediction
for these miRNAs, and a total of 448 pearl millet mRNAs were identified as the targets. Among these target
mRNAs, 122 (~25%) encode transcription factors. A pathway analysis showed that differentially expressed
miRNAs and their target genes can regulate the auxin response pathway. Quantitative real-time PCR analysis
of miRNAs and their targets showed consistent expression patterns. These results suggest that miRNAs play
a role in salinity stress tolerance in pearl millet.
 
Publisher Elsevier
 
Date 2020-06
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11688/1/Shinde%20et%20al%202020.pdf
Shinde, H and Dudhate, A and Anand, L and Tsugama, D and Gupta, S K and Liu, S and Takano, T (2020) Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses. South African Journal of Botany (TSI), 132. pp. 395-402. ISSN 0254-6299