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Mechanical force antagonizes the inhibitory effects of RecX on RecA filament formation in Mycobacterium tuberculosis.

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Relation http://ir.cftri.com/14758/
http://doi.org/10.1093/nar/gku899
 
Title Mechanical force antagonizes the inhibitory effects of
RecX on RecA filament formation in Mycobacterium
tuberculosis.
 
Creator Shimin, Le
Chen, Hu
Zhang, Xinghua
Chen, Jin
Neelakanteshwar Patil, K.
 
Subject 29 Protein Chemistry
 
Description Efficient bacterial recombinational DNA repair involves rapid cycles of RecA filament assembly and
disassembly. The RecX protein plays a crucial inhibitory role in RecA filament formation and stability. As the broken ends of DNA are tethered during
homologous search, RecA filaments assembled at
the ends are likely subject to force. In this work, we
investigated the interplay between RecX and force
on RecA filament formation and stability. Using magnetic tweezers, at single molecular level, we found
that Mycobacterium tuberculosis (Mt) RecX could
catalyze stepwise de-polymerization of preformed
MtRecA filament in the presence of ATP hydrolysis at
low forces (
 
Date 2014
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14758/1/Nucleic%20Acids%20Research%2C%202014%2C%20Vol.%2042%2C%20No.%2019.pdf
Shimin, Le and Chen, Hu and Zhang, Xinghua and Chen, Jin and Neelakanteshwar Patil, K. (2014) Mechanical force antagonizes the inhibitory effects of RecX on RecA filament formation in Mycobacterium tuberculosis. Nucleic Acids Research, 42 (9). pp. 11993-11999.