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Phenolic Extract of Seagrass, Halophila ovalis Activates Intrinsic Pathway of Apoptosis in Human Breast Cancer (MCF-7) Cells.

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Relation http://ir.cftri.com/14759/
https://doi.org/10.1080/01635581.2020.1743874
 
Title Phenolic Extract of Seagrass, Halophila ovalis
Activates Intrinsic Pathway of Apoptosis in Human
Breast Cancer (MCF-7) Cells.
 
Creator Harshita, Sharma
Nimish Mol, Stephen
Sowmyashree, G.
Deepika, U. R.
Yogendra Prasad, K.
Arpitha, H. S.
Hithamani, G.
Ganesan, P.
 
Subject 09 Human Physiology
Fish
 
Description The marine ecosystem is considered as a treasure of numerous novel biologically active
molecules. We investigated the anticancer potential of the phenolic extract of Halophila ovalis in breast cancer (MCF-7) cells and characterized the possible underlying molecular mechanism. The phenolic extract (5 ml) of H. ovalis effectively inhibited the growth of MCF-7 cells.
The results of DAPI staining indicated that this phenolic extract potently induces apoptosis
in MCF-7 cells which was observed by increased chromatin condensation in the treated
cells. An increased expression of the active fragments of an executioner caspase, caspase 3
in phenolic extract-treated MCF-7 cells further confirms this apoptosis induction. In consequence, the loss of mitochondrial membrane potential was noticed in treated cells. The protein expression analyzes show decreased expression of the anti-apoptotic protein, Bcl-2, and
DNA repair enzyme, PARP in treated cells indicating the probable molecular targets of apoptosis. Further, the phenolic extract of H. ovalis blocked the antioxidant defense system in
MCF-7 cells by down-regulating the protein expression of a major transcription factor, Nrf-2
and regulatory antioxidant enzymes, SOD-2 and HO-1. These results show the presence of
chemopreventive compound(s) in the phenolic extract, which offers a platform for future
studies to identify the active principles.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14759/1/NUTRITION%20AND%20CANCER%202020.pdf
Harshita, Sharma and Nimish Mol, Stephen and Sowmyashree, G. and Deepika, U. R. and Yogendra Prasad, K. and Arpitha, H. S. and Hithamani, G. and Ganesan, P. (2020) Phenolic Extract of Seagrass, Halophila ovalis Activates Intrinsic Pathway of Apoptosis in Human Breast Cancer (MCF-7) Cells. Nutrition and Cancer. pp. 1-11.