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Activity‑based protein profling of rice (Oryza sativa L.) bran serine hydrolases.

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Relation http://ir.cftri.com/14765/
https://doi.org/10.1038/s41598-020-72002-w
 
Title Activity‑based protein profling
of rice (Oryza sativa L.) bran serine
hydrolases.
 
Creator Achintya Kumar, Dolui
Arunkumar, V.
Ram, Rajasekharan
Vijayaraj, P.
 
Subject 07 Enzyme Biochemistry
07 Waste utilization
01 Rice
 
Description Rice bran is an underutilized agricultural by-product with economic importance. The unique
phytochemicals and fatty acid compositions of bran have been targeted for nutraceutical
development. The endogenous lipases and hydrolases are responsible for the rapid deterioration
of rice bran. Hence, we attempted to provide the frst comprehensive profling of active serine
hydrolases (SHs) present in rice bran proteome by activity-based protein profling (ABPP) strategy.
The active site-directed fuorophosphonate probe (rhodamine and biotin-conjugated) was used for
the detection and identifcation of active SHs. ABPP revealed 55 uncharacterized active-SHs and are
representing fve diferent known enzyme families. Based on motif and domain analyses, one of the
uncharacterized and miss annotated SHs (Os12Ssp, storage protein) was selected for biochemical
characterization by overexpressing in yeast. The purifed recombinant protein authenticated the
serine protease activity in time and protein-dependent studies. Os12Ssp exhibited the maximum
activity at a pH between 7.0 and 8.0. The protease activity was inhibited by the covalent serine
protease inhibitor, which suggests that the ABPP approach is indeed reliable than the sequence-based
annotations. Collectively, the comprehensive knowledge generated from this study would be useful
in expanding the current understanding of rice bran SHs and paves the way for better utilization/
stabilization of rice bran.
 
Date 2020
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14765/1/Scientific%20Reports%20%20%282020%29%201015191.pdf
Achintya Kumar, Dolui and Arunkumar, V. and Ram, Rajasekharan and Vijayaraj, P. (2020) Activity‑based protein profling of rice (Oryza sativa L.) bran serine hydrolases. Scientific Reports, 10. p. 15191.