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Parametric studies on batch degradation of a plasticizer di-n-butylphthalate by immobilized Bacillus sp.

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Relation http://ir.cftri.com/14770/
http://doi.org/10.1007/s11274-005-7369-0
 
Title Parametric studies on batch degradation of a plasticizer di-n-butylphthalate
by immobilized Bacillus sp.
 
Creator Neelakanteshwar Patil, K.
Karegoudar, T. B.
 
Subject 24 Organic Chemistry
02 Bacteriology
 
Description Di-n-butylphthalate (DBP) is one of the phthalate esters (PAEs) used in the manufacture of plasticizers, insect
repellents and synthetic fibres and contributes to environmental pollution. We report a novel bacterium belonging
to the genus, Bacillus (NCIM 5220), which has the ability to utilize DBP as the sole source of carbon and energy.
This bacterium was immobilized in alginate. The degradation of DBP by immobilized cells was compared with free
cells. The effects on the degradation of DBP of different factors like gel (alginate) concentration, gel bead size,
temperature, and pH were investigated. Oxygen uptake in the presence of DBP by free and immobilized cells was
also studied. The results showed that the degradation of DBP by immobilized cells was more efficient than by free
cells. Further, the effect of various factors tested on the degradation of DBP by alginate-immobilized cells showed
that the degradation of DBP was remarkably affected by alginate concentration between 2 and 5% and drastically
decreased between bead size 2 and 5 mm. A change of 10 �C of reaction temperature from 30 to 40 �C did not alter
the degradation of DBP, and maximum degradation was appeared to be favoured over a broad pH range of 6.5–7.5
for immobilized cells as compared to free cells, which showed an optimum temperature of about 35 �C and pH of
7.0. The immobilized cells showed higher oxidation of DBP than free cells. Thus more efficient degradation of DBP
could be achieved by immobilizing Bacillus sp. in alginate beads
 
Date 2005
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14770/1/World%20Journal%20of%20Microbiology%20%26%20Biotechnology%20%282005%29%20211493%E2%80%931498.pdf
Neelakanteshwar Patil, K. and Karegoudar, T. B. (2005) Parametric studies on batch degradation of a plasticizer di-n-butylphthalate by immobilized Bacillus sp. World Journal of Microbiology & Biotechnology, 21. pp. 1493-1498.