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Enhanced production of novel halostable recombinant endoglucanase derived from the metagenomic library using fed-batch fermentation.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Enhanced production of novel halostable recombinant endoglucanase derived from the metagenomic library using fed-batch fermentation.
 
Creator Kumar, Narender
Sudan, Sarabjeet Kour
Garg, Roma
Sahni, Girish
 
Subject QR Microbiology
 
Description Novel endoglucanase was isolated from soil metagenomic library.
40 fold increase in yield was obtained using fed- batch strategies as compared to shake flask.

Purified endogluconase was halostable and halotolerant as it retained 100% activity after 30 days incubation in presence of salts.

The purified enzyme was also found to be stable in presence of ions, organic solvents, surfactant and detergents.
 
Publisher Elsevier Science
 
Date 2019-05
 
Type Article
PeerReviewed
 
Relation https://www.sciencedirect.com/science/article/abs/pii/S1359511318315484
http://crdd.osdd.net/open/2640/
 
Identifier Kumar, Narender and Sudan, Sarabjeet Kour and Garg, Roma and Sahni, Girish (2019) Enhanced production of novel halostable recombinant endoglucanase derived from the metagenomic library using fed-batch fermentation. Process Biochemistry, 78. pp. 1-7. ISSN 13595113