Record Details

Microbacterium enclense sp nov., isolated from sediment sample

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Microbacterium enclense sp nov., isolated from sediment sample
 
Creator Mawlankar, Rahul
Mual, Poonam
Sonalkar, Vidya V
Thorat, Meghana N
(Verma, Ashish
Srinivasan, Krishnamurthi
Dastager, Syed G
 
Subject QR Microbiology
 
Description A novel bacterium (strain NIO-1002(T)) belonging to the genus Microbacterium was isolated from a marine sediment sample in Chorao Island, Goa Province, India. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Cells of this strain were Gram-stain-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. It grew in 0-12 % (w/v) NaCl and at 25-37 degrees C, with optimal growth at 30 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1002(T) is associated with members of the genus Microbacterium, with highest sequence similarity with Microbacterium hominis CIP 105731(T) (98.1%) and Microbacterium testaceum KCTC 9103(T) (98.0 %). Within the phylogenetic tree, this novel strain shared a branching point with M. hominis CIP 105731(T). The DNA G+C content was 66.5 mol% and DNA DNA hybridization relatedness between NI-1002(T), M. hominis CIP 105731(T) and M. testaceum KCTC 9103(T) was 39.0 +/- 2.0 % and 41.0 +/- 2.0 %, respectively. The major fatty acids were ai-C-15:0, i-C-16:0 and al-C-17:0 and the diamino acid in the cell-wall peptidoglycan of NIO-1002(T) was lysine. Data obtained from DNA-DNA hybridization and chemotaxonomic phenotypic analysis support the conclusion that strain NIO-1002(T) represents a novel species within the genus Microbacterium. The name Microbacterium enclense sp. nov. is proposed, with NIO-1002(T) (=NCIM 5454(T)=DSM 25125(T)=CCTCC AB 2011120(T)) as the type strain.
 
Publisher International Union of Microbiological Societies
 
Date 2019-05
 
Type Article
PeerReviewed
 
Identifier Mawlankar, Rahul and Mual, Poonam and Sonalkar, Vidya V and Thorat, Meghana N and (Verma, Ashish and Srinivasan, Krishnamurthi and Dastager, Syed G (2019) Microbacterium enclense sp nov., isolated from sediment sample. International journal of systematic and evolutionary microbiology, 69 (5). p. 1528. ISSN 1466-5034
 
Relation http://crdd.osdd.net/open/2644/