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Characterization of novel regulators for heat stress tolerance in tomato from Indian sub-continent

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Title Characterization of novel regulators for heat stress tolerance in tomato from Indian sub-continent
 
Creator Balyan, Sonia
Rao, Sombir
Jha, Sarita
Bansal, Chandni
Das, Jaishri Rubina
Mathur, Saloni
 
Subject Heat
tomato cultivar
tolerant
CLN1621L
CA4
transcriptome
 
Description Accepted date: 12 March 2020
The footprint of tomato cultivation, a cool region crop that exhibits heat stress (HS) sensitivity, is increasing in the tropics/sub‐tropics. Knowledge of novel regulatory hot‐spots from varieties growing in the Indian sub‐continent climatic zones could be vital for developing HS‐resilient crops. Comparative transcriptome‐wide signatures of a tolerant (CLN1621L) and sensitive (CA4) cultivar‐pair shortlisted from a pool of varieties exhibiting variable thermo‐sensitivity using physiological, survival and yield‐related traits revealed redundant to cultivar‐specific HS‐regulation. The antagonistically‐expressing genes encode enzymes and proteins that have roles in plant defense and abiotic stresses. Functional characterization of three antagonistic genes by overexpression and silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis), that are up‐regulated in tolerant cultivar, as positive regulators of HS‐tolerance and Solyc03g020030 (Pin‐II proteinase inhibitor), that is down‐regulated in CLN1621L, as negative regulator of thermotolerance. Transcriptional assessment of promoters of these genes by SNPs in stress‐responsive cis‐elements and promoter swapping experiments in opposite cultivar background showed inherent cultivar‐specific orchestration of transcription factors in regulating transcription. Moreover, overexpression of three ethylene response transcription factors (ERF.C1/F4/F5) also improved HS‐tolerance in tomato. This study identifies several novel HS‐tolerance genes and provides proof of their utility in tomato thermotolerance.
This work is supported by grants from NIPGR and DST-SERB, India (Project No.:
EMR/2016/006229). The authors acknowledge phytotron facility, CIF and field area
provided by NIPGR. The authors are thankful to DBT-eLibrary Consortium (DeLCON) for
providing access to e-resources. CLN1621L and CA4 seeds were provided by AVRDC,
Taiwan. SR acknowledges Department of Biotechnology (DBT) Govt. of India, SJ and CB
acknowledge University Grants Commission (UGC) and JRD acknowledges Council of
Scientific and Industrial Research (CSIR) for the award of research fellowships.
 
Date 2020-03-16T09:54:56Z
2020-03-16T09:54:56Z
2020
 
Type Article
 
Identifier Plant Biotechnology Journal, 18: 2118-2132
1467-7652
https://doi.org/10.1111/pbi.13371
https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13371
http://223.31.159.10:8080/jspui/handle/123456789/1048
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons