Record Details

Silencing AC1 of Tomato leaf curl virus using artificial microRNA confers resistance to leaf curl disease in transgenic tomato

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Silencing AC1 of Tomato leaf curl virus using artificial microRNA confers resistance to leaf curl disease in transgenic tomato
 
Creator Sharma, Namisha
Prasad, Manoj
 
Subject Artifcial microRNA
Tomato leaf curl disease
Tomato leaf curl New Delhi virus
Virus resistance
Replication initiator protein
 
Description Accepted date: 20 August 2020
Key message:
Expression of artificial microRNA targeting ATP binding domain of AC1 in transgenic tomato confers resistance to Tomato leaf curl disease without impacting the yield of tomato.
Abstract:
Tomato curl leaf disease caused by Tomato leaf curl virus (ToLCV) is a key constraint to tomato cultivation worldwide. Engineering transgenic plants expressing artificial microRNAs (amiRNAs) against the AC1 gene of Tomato leaf curl New Delhi virus (ToLCNDV), which is important for virus replication and pathogenicity, would consequently confer virus resistance and reduce crop loss in the economically important crops. This study relates to an amiRNA developed on the sequence of Arabidopsis miRNA319a, targeting the ATP/GTP binding domain of AC1 gene of ToLCNDV. The AC1-amiR was found to regulate the abundance of AC1, providing an excellent strategy in providing defense against ToLCNDV. Transgenic lines over-expressing AC1-amiR, when challenged with ToLCNDV, showed reduced disease symptoms and high percentage resistance ranging between ∼ 40 and 80%. The yield of transgenic plants was significantly higher upon ToLCNDV infection as compared to the non-transgenic plants. Although the natural resistance resources against ToLCNDV are not available, this work streamlines a novel amiRNA-based mechanism that may have the potential to develop viral resistance strategies in tomato, apart from its normal symptom development properties as it is targeting the conserved region against which higher accumulation of small interfering RNAs (siRNA) occurred in a naturally tolerant tomato cultivar.
The authors’ work in the area of plant–virus
interaction is supported by the JC Bose Fellowship (JCB/2018/00000l)
from Science and Engineering Research Board (SERB), Govt. of India,
India and core grant of National Institute of Plant Genome Research
(NIPGR), New Delhi. The authors thank Dr. Muthamilarasan Mehanathan, School of Life Sciences, University of Hyderabad, India, for
critically reading the manuscript. The authors are also thankful to DBTeLibrary Consortium (DeLCON) for providing access to e-resources.
 
Date 2020-09-01T10:43:39Z
2020-09-01T10:43:39Z
2020
 
Type Article
 
Identifier Plant Cell Reports, 39: 1565–1579
1432-203X
https://doi.org/10.1007/s00299-020-02584-2
https://link.springer.com/article/10.1007/s00299-020-02584-2
http://223.31.159.10:8080/jspui/handle/123456789/1089
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG