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Emerging tools and paradigm shift of gene editing in cereals, fruits, and horticultural crops for enhancing nutritional value and food security

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Title Emerging tools and paradigm shift of gene editing in cereals, fruits, and horticultural crops for enhancing nutritional value and food security
 
Creator Tiwari, Manish
Trivedi, Prabodh Kumar
Pandey, Ashutosh
 
Subject CRISPR/Cas9
crop
DNA repair
DSB
gene editing
NHEJ
ribonucleoprotein
 
Description Accepted date: 10 October 2020
Gene editing using sequence‐specific nucleases, particularly CRISPR/Cas ribonucleoprotein, has drawn enormous attention in plant research in recent years. Nearly a decade ago, Cas9 protein was initially discovered for a role in adaptive immunity in bacteria. Owing to vast potential, a large number of reports came out in a short span of time, comprising the identification of Cas protein from different bacterial sources, new Cas9 variants with reduced off‐targets, multiplexing, base editing, prime editing, and RNA manipulation in plants. Studies revealed that CRISPR/Cas‐based gene editing can play a major role in ensuring food security via developing resilient commercial crops with improved yield and nutritional value. Use of the CRISPR/Cas9 system for creating mutation in genes and regulatory regions of promoter generated a number of alleles with variable phenotypes, which can serve as an excellent genetic resource in the breeding program. In this review, we provide a recent overview of state‐of‐art discoveries in the CRISPR/Cas system comprised of new Cas proteins, modifications of existing Cas9, refinements in CRISPR/Cas‐induced gene editing, applications, and outcome emphasizing on major cereals and horticultural crops. We also highlight the current global policy framework for the regulation of gene‐edited crops.
This work was supported by the core grant of National
Institute of Plant Genome Research and Department of
Science and Technology-SERB for Startup research grant to
AP. MT thanks to Department of Biotechnology, Govt. of
India for funding assistance in the form of Ramalingaswami
Fellowship (grant no. BT/HRD/35/02/2006). PKT acknowledges Department of Biotechnology, New Delhi for the financial support in form of TATA Innovation Fellowship.
The authors are thankful to DBT-eLibrary Consortium
(DeLCON) for providing access to e-resources.
 
Date 2021-01-20T08:56:21Z
2021-01-20T08:56:21Z
2021
 
Type Article
 
Identifier Food and Energy Security, (In Press)
2048-3694
https://doi.org/10.1002/fes3.258
https://onlinelibrary.wiley.com/doi/full/10.1002/fes3.258
http://223.31.159.10:8080/jspui/handle/123456789/1148
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons