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Host gamma aminobutyric acid metabolic pathway is involved in resistance against Rhizoctonia solani

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Title Host gamma aminobutyric acid metabolic pathway is involved in resistance against Rhizoctonia solani
 
Creator Rani, Mamta
Jha, Gopaljee
 
Subject Fungal pathogenesis
Necrotroph
Reactive oxygen species
Virus induced gene silencing
 
Description Accepted date: 15 Dec 2020
Rhizoctonia solani is a highly destructive necrotrophic fungal pathogen having a diverse host range, including rice and tomato. Previously R. solani infection in rice has been found to cause large-scale readjustment in host primary metabolism and accumulation of various stress associated metabolites such as gamma aminobutyric acid (GABA). In this study, we report upregulation of GABA shunt genes during pathogenesis of R. solani in rice as well as tomato. The exogenous application of GABA provided partial resistance against R. solani infection in both the hosts. Further, using virus induced gene silencing (VIGS) approach, we knocked down the expression of some of the tomato genes involved in GABA biosynthesis (glutamate decarboxylase; GAD) and GABA catabolism (GABA-transaminase; GABA-T and succinic semialdehyde dehydrogenase; SSADH) to study their role in host defense against R. solani infection. The silencing of each of these genes was found to enhance disease susceptibility in tomato. Overall the results from gene expression analysis, exogenous chemical treatment and gene silencing studies suggest that GABA pathway plays a positive role in plant resistance against necrotrophic fungal pathogen R. solani.
This work was supported by NIPGR-flagship program entitled Imparting sheath blight
disease tolerance in rice, funded by DBT, Government of India. MR is supported by National
Post-Doctoral Fellowship from Science and Engineering Research Board, Government of India
(Govt. of India).
 
Date 2020-12-23T05:37:47Z
2020-12-23T05:37:47Z
2021
 
Type Article
 
Identifier Phytopathology, 111(7): 1207-1218
1943-7684
https://doi.org/10.1094/PHYTO-08-20-0356-R
https://apsjournals.apsnet.org/doi/10.1094/PHYTO-08-20-0356-R
http://223.31.159.10:8080/jspui/handle/123456789/1138
 
Language en_US
 
Format application/pdf
 
Publisher American Phytopathological Society