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Sucrose transport in response to drought and salt stress involves ABA-mediated induction of OsSWEET13 and OsSWEET15 in rice

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Title Sucrose transport in response to drought and salt stress involves ABA-mediated induction of OsSWEET13 and OsSWEET15 in rice
 
Creator Mathan, Jyotirmaya
Singh, Anuradha
Ranjan, Aashish
 
Subject rice
OsSWEET13 and OsSWEET15
Abiotic stresses
 
Description Accepted date: 07 September 2020
Abiotic stresses, including drought and salinity, negatively affect plant development and physiology at molecular and metabolic levels. Sucrose transport, mediating distribution of photosynthates in plant, is a key physiological process impacted by drought and salinity stresses, as sucrose is a prime energy and signaling molecule as well as an osmolyte. Therefore, understanding the effects of abiotic stresses on sucrose transport and transporters, and underlying genetic and molecular mechanisms is imperative to maintain sugar homeostasis in plants under stress. Here, we investigated the effects of drought and salinity stresses on sucrose transport and distribution, and on expression levels of genes encoding SWEET transporters, along with a potential transcription factor regulating SWEET expression in rice. We observed that drought and salinity stresses increased the sucrose content in leaf and root tissues, and in phloem sap of rice indica varieties. Expression analyses of SWEET genes and histochemical analysis of GUS reporter transgenic plants suggested that OsSWEET13 and OsSWEET15 are major SWEET transporters regulating the sucrose transport and levels in response to the abiotic stresses. Transactivation analyses showed that an ABA‐responsive transcription factor OsbZIP72 directly binds to the promoters of OsSWEET13 and OsSWEET15, and activates their expression. Taken together, the results showed that the higher expressions of OsSWEET13 and OsSWEET15 genes, induced by binding of an ABA‐responsive transcription factor OsbZIP72 to the promoters, potentially modulate sucrose transport and distribution in response to the abiotic stresses. The mechanism could possibly be targeted for maintaining sugar homeostasis in rice under drought and salinity stresses.
This work was supported by the Innovative Young Biotechnologist Award
(BT/09/IYBA/2015/01) from the Department of Biotechnology, Ministry of Science and Technology,
India and SERB-Early Career Research Award (ECR/2016/001588) from Science and Engineering
Research Board, India. JM and AS acknowledge their CSIR-JRF and SERB-NPDF fellowships,
respectively. We acknowledge Dr. Praveen Verma for generously providing Dual luciferase gateway
vector p635- REN-LUC, and Mr. Biswaranjan Rout for the technical help. We also acknowledge JNU
advanced instrumentation facility for mass spectrometry, NIPGR central instrumentation facility, and
DBT-eLibrary Consortium (DeLCON) for providing access to e-resources.
 
Date 2020-09-21T11:22:29Z
2020-09-21T11:22:29Z
2021
 
Type Article
 
Identifier Physiologia Plantarum, 171(4): 620-637
1399-3054
https://doi.org/10.1111/ppl.13210
https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.13210
http://223.31.159.10:8080/jspui/handle/123456789/1100
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons