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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responses

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Title Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responses
 
Creator Deb, Sohini
Ghosh, Palash
Patel, Hitendra K.
Sonti, Ramesh V.
 
Subject Xanthomonas oryzae pv. oryzae
14-3-3 protein
XopX
XopQ
resistance
rice
effector
 
Description Accepted date: 12 July 2020
Xanthomonas oryzae pv. oryzae uses several type III secretion system (T3SS) secreted effectors, namely XopN, XopQ, XopX, and XopZ, to suppress rice immune responses that are induced following treatment with cell wall degrading enzymes. Here we show that a T3SS secreted effector XopX interacts with two of the eight rice 14‐3‐3 proteins. Mutants of XopX that are defective in 14‐3‐3 binding are also defective in suppression of immune responses, suggesting that interaction with 14‐3‐3 proteins is required for suppression of host innate immunity. However, Agrobacterium mediated delivery of both XopQ and XopX into rice cells results in induction of rice immune responses. These immune responses are not observed when either protein is individually delivered into rice cells. XopQ‐ XopX induced rice immune responses are not observed with a XopX mutant that is defective in 14‐3‐3 binding. Yeast two‐ hybrid, BiFC and co‐ IP assays indicate that XopQ and XopX interact with each other. A screen for Xanthomonas effectors which can suppress XopQ‐ XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of Xanthomonas T3SS effectors in suppression of both pathogen triggered immunity and effector triggered immunity to promote virulence on rice.
This work was supported by grants to RVS from the Plant-Microbe and Soil Interaction (PMSI)
project of the Council of Scientific and Industrial Research (CSIR) (BSC0117), Government of
India and the J. C. Bose fellowship to RVS from the Department of Science and Technology
(DST), Government of India (GAP0444). This work was also supported by grants to HKP from
the Council of Scientific and Industrial Research (CSIR), Government of India (MLP0121).
 
Date 2020-07-16T05:41:41Z
2020-07-16T05:41:41Z
2020
 
Type Article
 
Identifier Plant Journal, 104: 332-350
1365-313X
https://doi.org/10.1111/tpj.14924
https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14924
http://223.31.159.10:8080/jspui/handle/123456789/1076
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons