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Methods for estimation of aflatoxins: a critical appraisal.

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Title Methods for estimation of aflatoxins: a critical appraisal.
 
Creator Shantha, T
 
Subject 09 Food Microbiology
 
Description Methods for estimation and detection of aflatoxins in foods are reviewed, with emphasis on advantages and disadvantages of each technique. Aspects considered include: importance of analytical techniques for aflatoxins; principles of estimation; steps involved in detection methods (sampling, extraction, extract purification); and detection and estimation methods (including: TLC; spectrophotometry; comparison of visual, spectrophotometric and fluorodensitometric estimation of aflatoxins; HPLC; immunoassay; preparation of aflatoxin B1 oxime; conjugation of the oxime to protein by mixed anhydride; production of antibodies against aflatoxins; development of immunoassay protocol; radioimmunoassay; ELISA; dot binding of antigens to nitrocellulose; quick screening test; advantages and limitation of immunoassays).
 
Date 1994
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/7909/1/JFST%201994%2091.pdf
Shantha, T (1994) Methods for estimation of aflatoxins: a critical appraisal. Journal of Food Science and Technology, India, 31 (2). 91-103, many ref..