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Optimisation and evaluation of cryopreservation method for Aurantiochytrium limacinum.

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Relation http://ir.cftri.com/14777/
https://doi.org/10.1007/s10811-020-02346-3
 
Title Optimisation and evaluation of cryopreservation method
for Aurantiochytrium limacinum.
 
Creator Ayushi, Dalmia
Ajay, W. Tumaney
 
Subject 05 Marine Biology
11 Lipid Biochemistry
 
Description Marine microorganisms are gaining increased global importance owing to their richness in various bioactive compounds. The
thraustochytrid Aurantiochytrium limacinum produces high amounts of the omega-3 fatty acid, DHA, and thus is of biotechnological importance. Aurantiochytrium limacinum cultures are usually maintained by continuous sub-culturing or plating, which is
labour and resource-intensive. Having a cryopreservation technique will be useful for the preservation of A. limacinum cultures.
Hence, we explored and developed a cryopreservation technique to preserve A. limacinum. Cryopreservation was done at two
different temperatures by using a mechanical freezer (− 80 °C) and liquid nitrogen (− 196 °C). In the mechanical freezer, the cells
were preserved in glycerol, while in liquid nitrogen four widely used cryoprotectants namely ethylene glycol, glycerol, methanol
and dimethyl sulfoxide were used. The cells stored in a mechanical freezer had a relatively constant growth rate up to 12 months
of storage. For liquid nitrogen storage, glycerol, methanol and dimethyl sulfoxide showed promising cryoprotectant activity with
viability as high as 80% even after 12 months of storage. However, ethylene glycol did not exhibit any cryoprotective effect
evidenced by marginal viability. Post-thaw assessment is crucial for judging the success of cryopreservation protocol and
therefore, the study also looked into the functionality of cells on revival. On reviving no significant difference in the fatty acid
profile and cell morphology among treatments was observed. The cryopreservation technique thus developed for preserving
A. limacinum will help save human efforts and time and also conserve the organism’s genetic integrity.
 
Date 2021
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14777/1/J%20Appl%20Phycol%202020.pdf
Ayushi, Dalmia and Ajay, W. Tumaney (2021) Optimisation and evaluation of cryopreservation method for Aurantiochytrium limacinum. Journal of Applied Phycology.