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Obestatin and Rosiglitazone Differentially Modulate Lipid Metabolism Through Peroxisome Proliferator-activated Receptor-γ (PPARγ) in Pre-adipose and Mature 3T3-L1 Cells.

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Relation http://ir.cftri.com/14778/
https://doi.org/10.1007/s12013-020-00958-7
 
Title Obestatin and Rosiglitazone Differentially Modulate Lipid
Metabolism Through Peroxisome Proliferator-activated Receptor-γ
(PPARγ) in Pre-adipose and Mature 3T3-L1 Cells.
 
Creator Mallikarjuna, B. G.
Uma, V. Manjappara
 
Subject 11 Lipid Biochemistry
 
Description Obestatin is a 23-residue peptide, obtained after posttranslational modification of preproghrelin. It has been shown, in
Swiss albino mice, to upregulate glycerolipid metabolism and PPARγ signaling. It was opined that the by-products of
increased glycerolipid metabolism triggered PPARγ signaling. It was hypothesized that obestatin upon co-administration
with a full agonist of PPARγ should reveal the comparative significance or possible synergy in PPARγ signaling. We
postulated they would act synergistically by obestatin increasing PPARγ expression and rosiglitazone enhancing PPARγ
activity. We evaluated the combination in DIO-C57BL/6 mice and observed that obestatin completely reversed the
increase in subcutaneous fat brought about by rosiglitazone. To understand their role at the adipocyte level, 3T3-L1 cells
were treated with a combination of obestatin and rosiglitazone during (1) initiation of differentiation and (2) after 14 days
from initiation of differentiation when the adipocytes were mature. Interestingly, their influence was mainly adipogenic
and showed double lipid accumulation when estimated 14 days after initiation of differentiation. There was an
upregulation of Pparγ by fourfold, Hsl by eightfold, Glut4 by fourfold, Leptin by 2.7-fold, Atgl by sixfold, Fasn by
sixfold, and Fabp4 by sevenfold at the mRNA level, whereas in mature adipocytes there was a significant decrease in fat
accumulation by 20%. There was downregulation of Pparγ, Hsl, Lpl, and Fasn by 0.5-fold at the mRNA level. These
results show that the combined influence of obestatin and rosiglitazone is significant and the outcome is dependent on the
metabolic stage of the adipocyte
 
Date 2021
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14778/1/Cell%20Biochemistry%20and%20Biophysics%202020.pdf
Mallikarjuna, B. G. and Uma, V. Manjappara (2021) Obestatin and Rosiglitazone Differentially Modulate Lipid Metabolism Through Peroxisome Proliferator-activated Receptor-γ (PPARγ) in Pre-adipose and Mature 3T3-L1 Cells. Cell Biochemistry and Biophysics.