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<p><strong>Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract</strong></p>

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Title Statement <p><strong>Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract</strong></p>
 
Added Entry - Uncontrolled Name Tamizhselvi, Ramasamy
Velusamy, Ranjith Kumar
Manickam, Venkatraman
Sirajudeen, Kuttulebbai NS
 
Uncontrolled Index Term Anti-inflammatory, Ayurvedic, Chandraprabha vati, Herbal, IкBα, Inflammation, Lipopolysaccharide, NF-кB activation, TNF-α, Siddha
 
Summary, etc. <p class="Abstract" style="text-align: justify;">The polyherbal formulation chandraprabha vati (CV) comprising 29 herbs is traditionally used as an anti-inflammatory agent for arthritis and urinary ailments in Indian Siddha medicine. Earlier, we reported that lipopolysaccharide (LPS) induced apoptosis in RAW 264.7 macrophages is mediated by hydrogen sulfide (H<sub>2</sub>S), a potential target for many inflammatory diseases. Here, we hypothesized that pretreatment with CV decreases H<sub>2</sub>S level and thereby alleviate the inflammatory conditions induced by LPS in RAW 264.7 macrophages, and this protective effect occurs through alterations in cystathionine-γ-lyase (CSE), a H<sub>2</sub>S synthesizing enzyme. Accordingly, we evaluated the anti-inflammatory effect of CV in LPS-stimulated RAW 264.7 macrophages <em>in vitro</em>. The CV pretreatment followed by 12 h of LPS-stimulation showed significantly decreased TNF-α, H<sub>2</sub>S production possibly through CSE gene expression and NF-кB activation compared to the non pretreated macrophages. Our results further confirm that the polyherbal extract chandraprabha vati may be a useful therapy for inflammatory disorders by reducing H<sub>2</sub>S levels.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-02-01 10:54:30
 
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http://op.niscair.res.in/index.php/IJEB/article/view/45723
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 02 (2021): IJEB [February 2021]
 
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