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<p><strong>Effect of Quercetin loaded Silver nanoparticles on Gram negative and Gram positive bacteria</strong></p>

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Title Statement <p><strong>Effect of Quercetin loaded Silver nanoparticles on Gram negative and Gram positive bacteria</strong></p>
 
Added Entry - Uncontrolled Name Saha, Chabita
Das, Asmita
Das, Pratyusa
Chakraborty, Aphrodite
 
Uncontrolled Index Term Catalase inhibition, DNA degradation, Mitochondrial membrane potential, Reactive oxygen species
 
Summary, etc. <p class="Abstract" style="text-align: justify;">Microorganisms are increasingly becoming resistant to multiple antibiotics and search for effective antibacterial agents continue to challenge researchers in the respective domain. Silver nanoparticles (AgNPs) with physicochemical properties different from their bulk counterparts and show better cellular penetration resulting in higher antimicrobial activity. Here, we studied the antimicrobial activity of AgNPs in pure state as well as when coated with quercetin, and also the mechanism of action. Quercetin is known for its antimicrobial activity, hence applied here for coating it on AgNPs. Spherical AgNPs of size ranging between 10 and 28 nm were synthesised using chemical reduction method. The AgNPs show promising antimicrobial activity in Gram negative bacteria <em>Escherchia coli</em>; however, AgNPs loaded with quercetin did not enhance the antimicrobial activity of AgNPs. The mechanism of antimicrobial activity of AgNPs unravelled in this study was inhibition of bacterial catalase and decrease in membrane potential (MMP) of the bacterial cell with increase in concentration of AgNPs. Another mechanistic aspect was production of reactive oxygen species (ROS) by AgNPs resulting in apoptosis. However, there was no degradation of cellular DNA which suggests that it does not contribute to antibacterial activity of AgNPs.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-02-01 10:54:30
 
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http://op.niscair.res.in/index.php/IJEB/article/view/45727
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 02 (2021): IJEB [February 2021]
 
Language Note en