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Development and validation of a novel RP-HPLC method for the simultaneous quantification of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol in a polyherbal formulation

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Title Development and validation of a novel RP-HPLC method for the simultaneous quantification of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol in a polyherbal formulation
 
Creator Kshirsagar, Rishikesh Rajendra
Vaidya, Shivani Atul
Jain, Vandana
 
Subject ICH
Phytoconstituents
Polyherbal formulation
RP-HPLC
Validation
 
Description 307-311
A novel, accurate, precise, and linear RP-HPLC method for the simultaneous estimation of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol in a polyherbal formulation was developed and validated as per ICH guidelines. A good chromatographic separation was achieved using gradient profile with the help of Shim-pack RP-HPLC column (250 mm*4.6 mm, 5 µm) and a mobile phase consisting of acetonitrile and 1% acetic acid was used. The column temperature was maintained at 28 °C throughout the run with a wavelength of 272 nm using UV-Visible detector. The retention time of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol was found to be 3.9, 6.7, 21.5, 44.6, and 45.9 min respectively. The linearity of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol was found to be in a range of 10-100, 5-50, 1-10, 5-50, and 2-20 µg/mL, respectively with correlation coefficient >0.99. The high recovery values (98-102%) indicate satisfactory accuracy. The % RSD values were found to be less than 2% in the precision study which reveals that the method is precise. Hence the developed method can be used for quality control and quantitative analysis of extracts and commercial containing these selected phytoconstituents.
 
Date 2021-02-04T09:09:00Z
2021-02-04T09:09:00Z
2020-12
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscair.res.in/handle/123456789/56141
 
Language en_US
 
Relation Int. cl. (2015.01)- A61K 36/00
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJNPR Vol.11(4) [December 2020]