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Suppression of pro-inflammatory cytokines and mediators production by ginger (Zingiber officinale Roscoe) ethanolic extract and gingerol in lipopolysaccharide-induced RAW 264.7 murine macrophage cells

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Title Suppression of pro-inflammatory cytokines and mediators production by ginger (Zingiber officinale Roscoe) ethanolic extract and gingerol in lipopolysaccharide-induced RAW 264.7 murine macrophage cells
 
Creator Saanin, Sri Nadya
Wahyudianingsih, Roro
Afni, Merry
Afifah, Ervi
Maesaroh, Maesaroh
Widowati, Wahyu
 
Subject Cytokines
Gingerol
Inflammation
Macrophage
Zingiber officinale
 
Description 260-266
Chronic inflammation could lead to several life-threatening diseases such as cancer and cardiovascular diseases. Ginger (Zingiber officinale Roscoe) has been used for many years to treat various diseases and health problems, including inflammation. This study was conducted to assess ginger ethanolic extract (GEE) and its compound gingerol’s potential as an anti-inflammatory agent by evaluating the concentration of pro-inflammatory cytokines and mediators such as TNF-α, IL-1β, IL-6, COX-2, and NO in LPS-induced RAW 264.7 cells. The safe concentration of GEE and gingerol for the RAW 264.7 cells were evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. The quantification of TNF-α, IL-1β, IL-6, COX-2, was conducted based on the ELISA method, while the quantification of NO was conducted by the nitrate/nitrite colourimetric method. The results showed that GEE and gingerol were able to inhibit TNF-α, IL-1β, IL-6, COX-2, and NO production in LPS-induced RAW 264.7 cells. GEE has better anti-inflammatory activity than gingerol. GEE in the concentration of 50 µg/mL has the highest inhibition activity over positive control or inflammatory cells model. GEE exhibited good anti-inflammatory properties through reduction of pro-inflammatory mediators such as TNF-α, IL-1β, IL-6, COX-2 and NO. Thus, ginger ethanolic extract has a high potential in the treatment of inflammation-related diseases.
 
Date 2021-02-04T09:20:51Z
2021-02-04T09:20:51Z
2020-12
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscair.res.in/handle/123456789/56146
 
Language en_US
 
Relation Int. cl. (2015.01)- A61K 36/00, A61K 36/906, A61K 125/00, A61P 29/00
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJNPR Vol.11(4) [December 2020]