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In vitro anticancer activity of silver nanoparticle synthesized from Punica granatum dried peel against cancer cell lines

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Title In vitro anticancer activity of silver nanoparticle synthesized from Punica granatum dried peel against cancer cell lines
 
Creator Paarakh, M. Padmaa
Jose, Preethy Ani
 
Subject Apoptosis
Cell Lines
Cell cycle
In vitro anticancer activity
Punica granatum
Silver nanoparticle
 
Description 224-238
The main aim of the investigation was to screen the silver and gold nanoparticle synthesized from the methanol concentrate of Punica granatum for its in vitro anticancer activity against MCF 2, PC3, A-549, HeLa, and HepG2 cell lines. Silver and gold nanoparticles were prepared from methanol concentrate of P. granatum and nanoparticle synthesized was analyzed by UV and TEM analysis. The impact of nanoparticles synthesized on MCF-2, PC3, A-549, HeLa, and HepG2 disease cell lines was assessed by MTT colourimetric assay and the impact on cell cycle was assessed by flow cytometric method. After assessing the cytotoxicity effect, the impact of apoptosis was also analyzed. The TEM analysis showed the particle size of 25.56 and 22.02 nm for gold and silver nanoparticles respectively. The adequacy of silver nanoparticles synthesized from P. granatum against MCF-2, PC3, A-549, HeLa, and HepG2 cell line demonstrated that the hatching of malignancy cells decreased the suitability of PC-3 and A 549 cancer cells lines only with IC50 values as 108.7 and 88.42 µg/mL respectively. Gold nanoparticles didn’t have any activity against all the cancer cell lines. Cell cycle analysis and apoptosis study showed that Silver nanoparticles are effective in controlling the cell cycle and blocking the apoptosis in all the cell lines used. P. granatum can be a very good anticancer drug for various cancer cell lines. All in all, P. granatum has a critical cell reinforcement movement and anticancer action.
 
Date 2021-02-04T09:37:27Z
2021-02-04T09:37:27Z
2020-12
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscair.res.in/handle/123456789/56150
 
Language en_US
 
Relation Int. cl. (2015.01)- A61K 36/00, A61K 131/00
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJNPR Vol.11(4) [December 2020]