Record Details

Deciphering the antagonistic effect of Streptomyces spp. and host-plant resistance induction against charcoal rot of sorghum

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/11704/
https://doi.org/10.1007/s00425-021-03577-5
doi:10.1007/s00425-021-03577-5
 
Title Deciphering the antagonistic effect of Streptomyces spp. and host-plant resistance induction against charcoal rot of sorghum
 
Creator Gopalakrishnan, S
Srinivas, V
Naresh, N
Pratyusha, S
Ankati, S
Madhuprakash, J
Govindaraj, M
Sharma, R
 
Subject Sorghum
Plant Disease
 
Description Two strains each of Streptomyces albus (CAI-17 and KAI-27) and Streptomyces griseus (KAI-26 and MMA-32)
and one strain of Streptomyces cavourensis (SAI-13) previously reported to have plant growth-promotion activity in chickpea,
rice and sorghum were evaluated for their antagonistic potential against Macrophomina phaseolina, which causes charcoal
rot in sorghum. The antagonistic potential of these strains against M. phaseolina was assessed through dual culture assay,
metabolite production assay, blotter paper assay in greenhouse and field disease screens. In both dual culture and metabolite
production assays, the selected strains significantly inhibited the growth of M. phaseolina (63–74%). In the blotter paper
assay, all the five strains of Streptomyces spp. inhibited the pathogen (80–90%). When these five strains were tested for their
antagonistic potential under the greenhouse (two times) and field (two seasons) conditions by toothpick method of inoculation,
significant differences were observed for charcoal rot severity. Principal component analysis capturing 91.3% phenotypic
variations, revealed that the shoot samples treated with both Streptomyces and the pathogen exhibited significantly enhanced
antioxidant parameters including superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione
reductase, phenylalanine ammonia-lyase, polyphenol oxidase, and total phenolic contents when compared to shoot samples
treated with only M. phaseolina. Scanning electron microscope analysis revealed that the phloem and xylem tissues of the
Streptomyces treated stem samples were intact compared to that of pathogen inoculated plants. This study indicated that the
selected strains of Streptomyces spp. have the potential for biological control of charcoal rot disease in sorghum.
 
Publisher Springer
 
Date 2021-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11704/1/host%E2%80%91plant%20resistance%20induction%20against%20charcoal%20rot%20of%20sorghum.pdf
Gopalakrishnan, S and Srinivas, V and Naresh, N and Pratyusha, S and Ankati, S and Madhuprakash, J and Govindaraj, M and Sharma, R (2021) Deciphering the antagonistic effect of Streptomyces spp. and host-plant resistance induction against charcoal rot of sorghum. Planta (TSI), 253 (2). pp. 1-12. ISSN 0032-0935