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Efficacy of seed defense proteins in biofortified pearl millet lines against blast and downy mildew

OAR@ICRISAT

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Relation http://oar.icrisat.org/11706/
https://doi.org/10.1007/s11738-021-03208-2
doi:10.1007/s11738-021-03208-2
 
Title Efficacy of seed defense proteins in biofortified pearl millet lines against blast and downy mildew
 
Creator Swathi, M
Naresh, N
Rani, T S
Govindaraj, M
Sharma, R
 
Subject Pearl Millet
Food and Nutrition
 
Description Pearl millet is a nutritious and climate-resilient dryland cereal crop. The present study was conducted to identify the defense
proteins in seed extrudes of 25 biofortified (Fe and Zn) inbred lines, and investigate their efficacy against blast (isolates Pg
45, Pg 138, and Pg 186) and downy mildew (isolates Sg 409, Sg 445, and Sg 576) diseases. The study revealed the presence
of cysteine protease inhibitors (4.3–58.5 units/mg) as well as pathogenesis-related (PR) hydrolases including chitinases
(5.3–16.3 units/ml), β-1,3-glucanases (7.3–32.9 units/mg), and cellulases (0.19–4.11 units/mg) in the test lines. The activity
levels of cystatins and PR hydrolases in the pearl millet lines were in relative consistency with the resistance levels observed
in the greenhouse screenings against blast and downy mildew diseases. Furthermore, antifungal screenings of seed proteins
against blast pathogen exhibited a significant reduction in radial growth of Pg 138 (57%) followed by Pg 186 (13%) and Pg
45 (10%). Spectrophotometric assays (
A595) exhibited significant retardation in spore germination and initial growth (48 h)
of Pg 45 (53.8–87.3%) followed by Pg 186 (19.2–61.3%) and Pg 138 (1.5–36.7%). Furthermore, seed proteins of biofortified
lines efficiently reduced the downy mildew disease incidence in greenhouse screenings by seed treatments of susceptible
pearl millet lines ICMP 451 (0.6–36% against Sg 409; 32–61% against Sg 576) and 7042R (14–80% against Sg 445). The
results of this study will provide insight into the biochemical basis of resistance in pearl millet against foliar blast and downy
mildew diseases, and to exploit novel strategies for breeding for disease resistance.
 
Publisher Springer
 
Date 2021-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11706/1/Swathi_et_al-2021-Acta_Physiologiae_Plantarum.pdf
Swathi, M and Naresh, N and Rani, T S and Govindaraj, M and Sharma, R (2021) Efficacy of seed defense proteins in biofortified pearl millet lines against blast and downy mildew. Acta Physiologiae Plantarum (TSI), 43 (3). pp. 1-15. ISSN 0137-5881