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Genotyping of cattle based on kappa-casein and alpha-lactalbumin genes

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Title Genotyping of cattle based on kappa-casein and alpha-lactalbumin genes
 
Creator Sumaiya, Rahman
Hossain, Md. Imam
Hossain, Mridha Md. Kamal
Alim, Md. Abdul
Nusrat, Jahan
Islam, Md. Ariful
Rubaya
Salimullah Md
Ara, Bhuyan Anjuman
Jahangir, Alam
 
Subject Cattle
Genotypes
Kappa-casein
Alpha - lactalbumin
Allele frequency
 
Description 211-218
Milk yield, protein and fat content are regulated by milk protein genes. However, genetic makeup of different milk protein genes of cattle available in Bangladesh is not known. This study determined the genetic variants of kappa-casein (κ-CN) and α-lactalbumin (α-LA) gene in cattle. DNA was extracted from 50 blood samples collected from indigenous and crossbred cattle. Polymerase chain reaction (PCR) was performed with gene specific primers. For genotyping PCR products of κ-casein gene was digested with HindIII and HaeIII restriction enzymes while the PCR products of α-LA gene was digested with Bsp1286I. Overall from all the tested samples we could amplify specific DNA viz. 935 and 429 bp for κ-CN and α-LA genes, respectively. In case of κ-CN gene 30 samples were analyzed for genotyping and of these 66.67, 30.00 and 3.33% samples were genotyped as AA, AB and BB, respectively. AA genotype was found dominant for κ-CN gene. Allele frequency for A was 82%. AA genotype was found higher in indigenous (0.366) than crossbred (0.30) animals. Homozygous BB genotype was not found in crossbred animals. For α-LA gene 50 samples were genotyped and all the samples (100%) were belonging to AA genotype. Three genetic variants of κ-CN gene viz. AA, AB & BB and one genotype (AA) of α-LA gene were detected in tested samples. Alleles of both the genes are reported to be associated with higher milk, protein, and fat yields.
 
Date 2021-02-12T04:51:12Z
2021-02-12T04:51:12Z
2020-07
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/56219
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBT Vol.19(3) [July 2020]