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In vivo genomic expression of luteinizing hormone receptors and steroidogenic-enzyme in follicles of crossbred cows

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Title In vivo genomic expression of luteinizing hormone receptors and steroidogenic-enzyme in follicles of crossbred cows
 
Creator Satheshkumar, S
 
Subject Ovarian granulosa cell
LH receptor
Steroidogenic enzyme
Indian crossbred cows
 
Description 206-210
The present work was aimed at documenting the in vivo expression patterns of luteinizing hormone receptor (LHr) and cytochrome P450 aromatase enzyme (CYP19) genes in the granulosa cells (GC) of dominant follicles (DF) in Indian crossbred cows. Six crossbred cows were utilized for the study. Ultrasound guided in vivo follicular aspiration was performed on the specific days of the cycle viz., Day 0-oestrus (preovulatory follicle-POF); Day 6-wave I growing DF (GDF) and Day 8-wave I regressing DF (RDF). Twelve follicles in each category were aspirated and GCs were separated. Qualitative and semi-quantitative analysis of LHr and CYP-19 genes in GCs of all the three categories of the DFs were studied by conventional polymerase chain reaction (PCR) and densitometry analysis. PCR studies showed that mRNA expression for the LHr was enhanced in GDF comparable with that of POF, but significantly reduced in RDF. Analysis of relative expression levels revealed that the LHr expression was reduced by 1.5 folds in Day 8 RDF when compared to GDF. The expression levels of CYP19 gene in POF were greater than GDF and RDF respectively. Increased expression levels of these genes explained the ovulatory nature of POF when compared to wave I GDF.
 
Date 2021-02-12T04:52:53Z
2021-02-12T04:52:53Z
2020-07
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/56220
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBT Vol.19(3) [July 2020]