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Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Fusarium udum Butler) Dependent on Genotype Variability in the Leguminous Crop Pigeonpea

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Relation http://oar.icrisat.org/11725/
https://doi.org/10.3389/fgene.2020.00862
doi:10.3389/fgene.2020.00862
 
Title Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Fusarium udum Butler) Dependent on Genotype Variability in the Leguminous Crop Pigeonpea
 
Creator Biswas, K
Tarafdar, A
Kumar, R
Singhvi, N
Ghosh, P
Sharma, M
Pabbi, S
Shukla, P
 
Subject Pigeonpea
Plant Pathology
Plant Disease
 
Description Fusarium wilt (FW), caused by Fusarium udum Butler (FU), is among the
challenging factors in the production of pigeonpea. Therefore, exploring a superior
pigeonpea genotype from landraces or local cultivars through the selection of
innate resistance to FW using different biological and molecular approaches, and
validating its resistance response, could be an alternative to sustainable crop
improvement. Five distinct pigeonpea genotypes, with resistant (ICP2894) and
susceptible (ICP2376) controls, were selected on the basis of the incidence percentage
of FW, from three different states of India. Among them, the cultivar Richa,
which displayed low incidence of FW (10.0%) during the genotype evaluation,
was further examined for its innate resistance to FW. Molecular characterization
of antioxidant (AO) enzyme [APX and SOD] and pathogenesis-related (PR) protein
[CHS and b-1, 3-glucanase] families were performed. The obtained results of
reverse transcription-polymerase chain reaction-based expression study and in silico
analysis showed a higher level of induction of PR and AO genes, and the
strong interaction of their putative proteins with fungal cellobiohydrolase-c protein
established their antifungal activity, conferring early plant defense responses to FU in Richa. Our study demonstrated a strong and combinatorial approach
involving biological assay, molecular experiments, and in silico analysis to identify
a superior pigeonpea genotype that was resistant to FW across a major
biogeographic region.
 
Publisher FRONTIERS MEDIA SA
 
Date 2020-08
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11725/1/fgene-11-00862.pdf
Biswas, K and Tarafdar, A and Kumar, R and Singhvi, N and Ghosh, P and Sharma, M and Pabbi, S and Shukla, P (2020) Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Fusarium udum Butler) Dependent on Genotype Variability in the Leguminous Crop Pigeonpea. Frontiers in Genetics (TSI), 11. pp. 1-15. ISSN 1664-8021